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BMRB: 17067

2L0Y

Complex hMia40-hCox17

Authors

Bertini, I., Ciofi-Baffoni, S., Gallo, A.

Assembly

Complex hMia40-hCox17

Entity

1. Mia40 (polymer, Thiol state: all disulfide bound), 146 monomers, 16372.56 Da Detail

GSFTMSYCRQ EGKDRIIFVT KEDHETPSSA ELVADDPNDP YEEHGLILPN GNINWNSPCL GGMASGPCGE QFKSAFSCFH YSTEEIKGSD CVDQFRAMQE CMQKYPDLYP QEDEDEEEER EKKPAEQAEE TAPIEATATK EEEGSS

2. Cox17 (polymer, Thiol state: all disulfide bound), 67 monomers, 7259.255 Da Detail

GSFTMPGLVD SNPAPPESQE KKPLKPCCAS PETKKARDAS IIEKGEEHCG HLIEAHKESM RALGFKI

Total weight

23631.814 Da

Max. entity weight

16372.56 Da

Entity Connection

disulfide 3 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS59:SG	2:CYS49:SG
2	disulfide	sing	1:CYS68:SG	1:CYS101:SG
3	disulfide	sing	1:CYS78:SG	1:CYS91:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 76.1 %, Completeness: 50.3 %, Completeness (bb): 70.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	50.3 % (1205 of 2395)	39.8 % (503 of 1263)	59.6 % (549 of 921)	72.5 % (153 of 211)
Backbone	70.4 % (876 of 1244)	61.1 % (259 of 424)	74.4 % (464 of 624)	78.1 % (153 of 196)
Sidechain	35.4 % (477 of 1349)	29.0 % (243 of 839)	47.3 % (234 of 495)	0.0 % (0 of 15)
Aromatic	8.3 % (13 of 156)	16.7 % (13 of 78)	0.0 % (0 of 77)	0.0 % (0 of 1)
Methyl	37.3 % (53 of 142)	11.3 % (8 of 71)	63.4 % (45 of 71)

1. Mia40

GSFTMSYCRQ EGKDRIIFVT KEDHETPSSA ELVADDPNDP YEEHGLILPN GNINWNSPCL GGMASGPCGE QFKSAFSCFH YSTEEIKGSD CVDQFRAMQE CMQKYPDLYP QEDEDEEEER EKKPAEQAEE TAPIEATATK EEEGSS

2. Cox17

GSFTMPGLVD SNPAPPESQE KKPLKPCCAS PETKKARDAS IIEKGEEHCG HLIEAHKESM RALGFKI

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Mia40	[U-100% 13C; U-100% 15N]	0.5 mM
2	Cox17	natural abundance	0.5 mM
3	Phosphate	natural abundance	50 mM
4	EDTA	natural abundance	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
7	Cox17	[U-100% 13C; U-100% 15N]	0.5 mM
8	Mia40	natural abundance	0.5 mM
9	Phosphate	natural abundance	50 mM
10	EDTA	natural abundance	0.5 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	Dioxane	methylene carbons	external	direct	1.0
¹H	TMS	methyl protons	external	direct	1.0
¹⁵N	TMS	methyl protons	external	indirect	0.1013291

Referencing offset: 2.76 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	Dioxane	methylene carbons	external	direct	1.0
¹H	TMS	methyl protons	external	direct	1.0
¹⁵N	TMS	methyl protons	external	indirect	0.1013291

Referencing offset: 2.76 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	Dioxane	methylene carbons	external	direct	1.0
¹H	TMS	methyl protons	external	direct	1.0
¹⁵N	TMS	methyl protons	external	indirect	0.1013291

Referencing offset: 3.11 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L0Y, Strand ID: A, B Detail

Release date

2010-11-14

Citation

Molecular chaperone function of Mia40 triggers consecutive induced folding steps of the substrate in mitochondrial protein import
Banci, L., Bertini, I., Cefaro, C., Cenacchi, L., Ciofi-Baffoni, S., Felli, I., Gallo, A., Gonnelli, L., Luchinat, E., Sideris, D., Tokatlidis, K.
Proc. Natl. Acad. Sci. U. S. A. (2010), 107, 20190-20195, PubMed 21059946 , DOI 10.1073/pnas.1010095107 , Abstract

Entries sharing articles Swiss-Prot: 2 entries Detail

  Swiss-Prot: Q8N4Q1 released on 2005-04-12
    Title MIA40_HUMAN Entity Mitochondrial intermembrane space import and assembly protein 40
  Swiss-Prot: Q14061 released on 1997-11-01
    Title COX17_HUMAN Entity Cytochrome c oxidase copper chaperone

Related entities 1. Mia40, : 2 : 9 entities Detail

Related entities 2. Cox17, : 1 : 5 : 15 entities Detail

Interaction partners 1. Mia40, : 7 : 1 interactors Detail

More details for 8 interactors identified by 6 citations

Interaction partners 2. Cox17, : 14 : 1 interactors Detail

More details for 15 interactors identified by 5 citations

Experiments performed 20 experiments Detail

1 2D 1H-15N HSQC