Mouse prion protein (121-231) with the mutations Y169A, Y225A, and Y226A.
GSVVGGLGGY MLGSAMSRPM IHFGNDWEDR YYRENMYRYP NQVYYRPVDQ ASNQNNFVHD CVNITIKQHT VTTTTKGENF TETDVKMMER VVEQMCVTQY QKESQAAADG RRSS
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS61:SG | 1:CYS96:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 89.2 % (1169 of 1311) | 97.8 % (668 of 683) | 74.8 % (374 of 500) | 99.2 % (127 of 128) |
Backbone | 83.3 % (565 of 678) | 95.7 % (224 of 234) | 69.4 % (231 of 333) | 99.1 % (110 of 111) |
Sidechain | 95.9 % (708 of 738) | 98.9 % (444 of 449) | 90.8 % (247 of 272) | 100.0 % (17 of 17) |
Aromatic | 79.7 % (94 of 118) | 98.3 % (58 of 59) | 60.3 % (35 of 58) | 100.0 % (1 of 1) |
Methyl | 97.8 % (90 of 92) | 97.8 % (45 of 46) | 97.8 % (45 of 46) |
1. Prion with Y169A, Y225A, Y226A mutation
GSVVGGLGGY MLGSAMSRPM IHFGNDWEDR YYRENMYRYP NQVYYRPVDQ ASNQNNFVHD CVNITIKQHT VTTTTKGENF TETDVKMMER VVEQMCVTQY QKESQAAADG RRSSSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.2 K, pH 4.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | prion | [U-99% 13C; U-99% 15N] | 1.4 mM | |
2 | sodium acetate | [U-2H] | 10 mM | |
3 | sodium azide | natural abundance | 0.02 % | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |