solution structure of a preQ1 riboswitch (Class I) aptamer bound to preQ1
Polymer type: polyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 92.1 % (527 of 572) | 93.2 % (289 of 310) | 93.1 % (229 of 246) | 56.3 % (9 of 16) |
Suger, PO4 | 93.7 % (371 of 396) | 94.9 % (205 of 216) | 92.2 % (166 of 180) | |
Nucleobase | 88.6 % (156 of 176) | 89.4 % (84 of 94) | 95.5 % (63 of 66) | 56.3 % (9 of 16) |
Aromatic | 89.0 % (146 of 164) | 90.2 % (74 of 82) | 95.5 % (63 of 66) | 56.3 % (9 of 16) |
1. RNA (36-MER)
GGAGAGGUUC UAGUUAUACC CUCUAUAAAA AACUAASolvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | preQ1-RNA 36mer | [U-13C; U-15N]-Cyt | 1 mM | |
2 | D2O | natural abundance | 100 % | |
3 | KCl | natural abundance | 50 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | preQ1-RNA 36mer | natural abundance | 1 mM | |
5 | H2O | natural abundance | 90 % | |
6 | D2O | natural abundance | 10 % | |
7 | KCl | natural abundance | 50 mM |
Solvent system 90% H2O/10% D2O, phage, Pressure 1 atm, Temperature 300 K, pH 6.3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | preQ1-RNA 36mer | [U-13C; U-15N] | 0.7 mM | |
9 | H2O | natural abundance | 90 % | |
10 | D2O | natural abundance | 10 % | |
11 | KCl | natural abundance | 50 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
12 | preQ1-RNA 36mer | [U-13C; U-15N]-Ade | 1 mM | |
13 | D2O | natural abundance | 100 % | |
14 | KCl | natural abundance | 50 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
15 | preQ1-RNA 36mer | [U-13C; U-15N]-Gua | 1 mM | |
16 | D2O | natural abundance | 100 % | |
17 | KCl | natural abundance | 50 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
18 | preQ1-RNA 36mer | [U-13C; U-15N]-Ura | 1 mM | |
19 | D2O | natural abundance | 100 % | |
20 | KCl | natural abundance | 50 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
21 | preQ1-RNA 36mer | U-H5,3',4',5',5''-2H | 1 mM | |
22 | D2O | natural abundance | 100 % | |
23 | KCl | natural abundance | 50 mM |