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BMRB: 17141

2L2M

Backbone 1H, 13C, and 15N Chemical Shift Assignments for HYL1-dsRBD2

Authors

Rasia, R.M., Mateos, J.L., Bologna, N.G., Burdisso, P., Imbert, L., Palatnik, J.F., Boisbouvier, J.

Assembly

HYL1 second dsRBD

Entity

1. HYL1 second dsRBD (polymer, Thiol state: all free), 77 monomers, 8463.675 Da Detail

GHMHETGLCK NLLQEYAQKM NYAIPLYQCQ KVETLGRVTQ FTCTVEIGGI KYTGAATRTK KDAEISAGRT ALLAIQS

Formula weight

8463.675 Da

Source organism

Arabidopsis thaliana

Exptl. method

Data set

assigned_chemical_shifts, RDCs

Chem. Shift Complete

Sequence coverage: 90.9 %, Completeness: 42.6 %, Completeness (bb): 73.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	42.6 % (375 of 880)	22.4 % (102 of 455)	60.7 % (207 of 341)	78.6 % (66 of 84)
Backbone	73.7 % (339 of 460)	46.3 % (74 of 160)	89.3 % (200 of 224)	85.5 % (65 of 76)
Sidechain	20.2 % (99 of 490)	9.5 % (28 of 295)	37.4 % (70 of 187)	12.5 % (1 of 8)
Aromatic	0.0 % (0 of 50)	0.0 % (0 of 25)	0.0 % (0 of 25)
Methyl	19.1 % (18 of 94)	14.9 % (7 of 47)	23.4 % (11 of 47)

1. hyl1-2

GHMHETGLCK NLLQEYAQKM NYAIPLYQCQ KVETLGRVTQ FTCTVEIGGI KYTGAATRTK KDAEISAGRT ALLAIQS

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	hyl1-2	[U-100% 13C; U-100% 15N]		0.8 mM
2	DTT	natural abundance		10 mM
3	sodium phosphate	natural abundance		100 mM
4	sodium chloride	natural abundance		50 mM
5	sodium azide	natural abundance		0.05 %
6	H2O	natural abundance		90 %
7	D2O	natural abundance		10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
8	hyl1-2	[U-100% 13C; U-100% 15N]		0.8 mM
9	DTT	natural abundance		10 mM
10	sodium phosphate	natural abundance		100 mM
11	sodium chloride	natural abundance		50 mM
12	sodium azide	natural abundance		0.05 %
13	C12E5	natural abundance		5 %
14	Hexanol	natural abundance		0.2 %
15	H2O	natural abundance		90 %
16	D2O	natural abundance		10 %

RDC

181 RDC values in 1 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 298 K, pH 7.0 Detail

Release date

2010-09-27

Citation

Structure and RNA interactions of the plant MicroRNA processing-associated protein HYL1
Rasia, R.M., Mateos, J., Bologna, N.G., Burdisso, P., Imbert, L., Palatnik, J.F., Boisbouvier, J.
Biochemistry (2010), 49, 8237-8239, PubMed 20735118 , DOI 10.1021/bi100672x , Abstract

Related entities 1. HYL1 second dsRBD, : 1 : 1 : 178 entities Detail

Interaction partners 1. HYL1 second dsRBD, : 7 interactors Detail

More details for 7 interactors identified by 3 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian DirectDrive - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	hyl1-2	[U-100% 13C; U-100% 15N]		0.8 mM
2	DTT	natural abundance		10 mM
3	sodium phosphate	natural abundance		100 mM
4	sodium chloride	natural abundance		50 mM
5	sodium azide	natural abundance		0.05 %
6	H2O	natural abundance		90 %
7	D2O	natural abundance		10 %

2 3D HNCO

Instrument

Varian DirectDrive - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	hyl1-2	[U-100% 13C; U-100% 15N]		0.8 mM
2	DTT	natural abundance		10 mM
3	sodium phosphate	natural abundance		100 mM
4	sodium chloride	natural abundance		50 mM
5	sodium azide	natural abundance		0.05 %
6	H2O	natural abundance		90 %
7	D2O	natural abundance		10 %

3 3D HNCA

Instrument

Varian DirectDrive - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	hyl1-2	[U-100% 13C; U-100% 15N]		0.8 mM
2	DTT	natural abundance		10 mM
3	sodium phosphate	natural abundance		100 mM
4	sodium chloride	natural abundance		50 mM
5	sodium azide	natural abundance		0.05 %
6	H2O	natural abundance		90 %
7	D2O	natural abundance		10 %

4 3D HNCACB

Instrument

Varian DirectDrive - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	hyl1-2	[U-100% 13C; U-100% 15N]		0.8 mM
2	DTT	natural abundance		10 mM
3	sodium phosphate	natural abundance		100 mM
4	sodium chloride	natural abundance		50 mM
5	sodium azide	natural abundance		0.05 %
6	H2O	natural abundance		90 %
7	D2O	natural abundance		10 %

5 3D HN(CO)CA

Instrument

Varian DirectDrive - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	hyl1-2	[U-100% 13C; U-100% 15N]		0.8 mM
2	DTT	natural abundance		10 mM
3	sodium phosphate	natural abundance		100 mM
4	sodium chloride	natural abundance		50 mM
5	sodium azide	natural abundance		0.05 %
6	H2O	natural abundance		90 %
7	D2O	natural abundance		10 %

6 3D HN(COCA)CB

Instrument

Varian DirectDrive - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	hyl1-2	[U-100% 13C; U-100% 15N]		0.8 mM
2	DTT	natural abundance		10 mM
3	sodium phosphate	natural abundance		100 mM
4	sodium chloride	natural abundance		50 mM
5	sodium azide	natural abundance		0.05 %
6	H2O	natural abundance		90 %
7	D2O	natural abundance		10 %

7 3D HN(CA)CO

Instrument

Varian DirectDrive - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	hyl1-2	[U-100% 13C; U-100% 15N]		0.8 mM
2	DTT	natural abundance		10 mM
3	sodium phosphate	natural abundance		100 mM
4	sodium chloride	natural abundance		50 mM
5	sodium azide	natural abundance		0.05 %
6	H2O	natural abundance		90 %
7	D2O	natural abundance		10 %

8 3D HNCO JCACO

Instrument

Varian DirectDrive - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	hyl1-2	[U-100% 13C; U-100% 15N]		0.8 mM
2	DTT	natural abundance		10 mM
3	sodium phosphate	natural abundance		100 mM
4	sodium chloride	natural abundance		50 mM
5	sodium azide	natural abundance		0.05 %
6	H2O	natural abundance		90 %
7	D2O	natural abundance		10 %

9 3D HNCO JNH

Instrument

Varian DirectDrive - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	hyl1-2	[U-100% 13C; U-100% 15N]		0.8 mM
2	DTT	natural abundance		10 mM
3	sodium phosphate	natural abundance		100 mM
4	sodium chloride	natural abundance		50 mM
5	sodium azide	natural abundance		0.05 %
6	H2O	natural abundance		90 %
7	D2O	natural abundance		10 %

10 3D HNCOCA JCAHA

Instrument

Varian DirectDrive - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	hyl1-2	[U-100% 13C; U-100% 15N]		0.8 mM
2	DTT	natural abundance		10 mM
3	sodium phosphate	natural abundance		100 mM
4	sodium chloride	natural abundance		50 mM
5	sodium azide	natural abundance		0.05 %
6	H2O	natural abundance		90 %
7	D2O	natural abundance		10 %

11 3D HNCO JCACO

Instrument

Varian DirectDrive - 600 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
8	hyl1-2	[U-100% 13C; U-100% 15N]		0.8 mM
9	DTT	natural abundance		10 mM
10	sodium phosphate	natural abundance		100 mM
11	sodium chloride	natural abundance		50 mM
12	sodium azide	natural abundance		0.05 %
13	C12E5	natural abundance		5 %
14	Hexanol	natural abundance		0.2 %
15	H2O	natural abundance		90 %
16	D2O	natural abundance		10 %

12 3D HNCO JNH

Instrument

Varian DirectDrive - 600 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
8	hyl1-2	[U-100% 13C; U-100% 15N]		0.8 mM
9	DTT	natural abundance		10 mM
10	sodium phosphate	natural abundance		100 mM
11	sodium chloride	natural abundance		50 mM
12	sodium azide	natural abundance		0.05 %
13	C12E5	natural abundance		5 %
14	Hexanol	natural abundance		0.2 %
15	H2O	natural abundance		90 %
16	D2O	natural abundance		10 %

13 3D HNCOCA JCAHA

Instrument

Varian DirectDrive - 600 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
8	hyl1-2	[U-100% 13C; U-100% 15N]		0.8 mM
9	DTT	natural abundance		10 mM
10	sodium phosphate	natural abundance		100 mM
11	sodium chloride	natural abundance		50 mM
12	sodium azide	natural abundance		0.05 %
13	C12E5	natural abundance		5 %
14	Hexanol	natural abundance		0.2 %
15	H2O	natural abundance		90 %
16	D2O	natural abundance		10 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts, RDC exptl data	bmr17141_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr17141_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	321		88.3 (chain: 1, length: 77)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 77, Sequence coverage: 88.3%
- Total number of assignments
  - ¹³C chemical shifts: 195
  - ¹⁵N chemical shifts: 63
  - ¹H chemical shifts: 63
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords dsRBD, miRNA