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DNA repair protein zinc finger 1
Authors
Neuhaus, D., Eustermann, S., Yang, J., Videler, H.
Assembly
PARP-1 Finger 1
Entity
1. PARP-1 Finger 1 (polymer, Thiol state: all other bound), 108 monomers, 12110.61 Da Detail

MAESSDKLYR VEYAKSGRAS CKKCSESIPK DSLRMAIMVQ SPMFDGKVPH WYHFSCFWKV GHSIRHPDVE VDGFSELRWD DQQKVKKTAE AGGVTGKGQD GIGSKAEK


2. ZN (non-polymer), 65.409 Da
Total weight
12176.02 Da
Max. entity weight
12110.61 Da
Entity Connection
na 4 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1nasing1:CYS21:SG2:ZN1:ZN
2nasing1:CYS24:SG2:ZN1:ZN
3nasing1:HIS53:ND12:ZN1:ZN
4nasing1:CYS56:SG2:ZN1:ZN

Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 97.2 %, Completeness: 88.3 %, Completeness (bb): 83.3 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All88.3 % (1107 of 1254)94.7 % (624 of 659)78.3 % (379 of 484)93.7 % (104 of 111)
Backbone83.3 % (533 of 640)97.3 % (216 of 222)68.8 % (216 of 314)97.1 % (101 of 104)
Sidechain94.0 % (669 of 712)93.4 % (408 of 437)96.3 % (258 of 268)42.9 % (3 of 7)
Aromatic100.0 % (116 of 116)100.0 % (58 of 58)100.0 % (55 of 55)100.0 % (3 of 3)
Methyl97.4 % (76 of 78)97.4 % (38 of 39)97.4 % (38 of 39)

1. PARP-1 Finger 1

MAESSDKLYR VEYAKSGRAS CKKCSESIPK DSLRMAIMVQ SPMFDGKVPH WYHFSCFWKV GHSIRHPDVE VDGFSELRWD DQQKVKKTAE AGGVTGKGQD GIGSKAEK

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 300 K, pH 7.0


#NameIsotope labelingTypeConcentration
1PARP-1 Finger 1[U-98% 15N; U-98% 13C]0.5 ~ 1.0 mM
2TRIS[U-99% 2H]50 mM
3sodium chloridenatural abundance200 mM
4zinc sulfatenatural abundance150 uM
5DTT[U-99% 2H]4 mM
6H2Onatural abundance95 %
7D2Onatural abundance5 %
8TRIS[U-99% 2H]50 mM

LACS Plot; CA
Referencing offset: 0.14 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 0.14 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.05 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 30 models in PDB: 2L30, Strand ID: A Detail


Release date
2011-01-26
Citation
The DNA-binding domain of human PARP-1 interacts with DNA single-strand breaks as a monomer through its second zinc finger
Eustermann, S., Videler, H., Yang, J., Cole, P.T., Gruszka, D., Veprintsev, D., Neuhaus, D.
J. Mol. Biol. (2011), 407, 149-170, PubMed 21262234 , DOI 10.1016/j.jmb.2011.01.034 ,
Related entities 1. PARP-1 Finger 1, : 1 : 3 : 7 : 44 entities Detail
Interaction partners 1. PARP-1 Finger 1, : 182 interactors Detail
Experiments performed 14 experiments Detail
nullKeywords protein, zinc finger