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BMRB: 17161

2L9B

Rna14p/Rna15p heterodimer

Authors

Mackereth, C.D.

Assembly

Rna14p/Rna15p

Entity

1. Rna14p (polymer, Thiol state: not present), 53 monomers, 6048.824 Da Detail

MKRDSELPTE VLDLLSVIPK RQYFNTNLLD AQKLVNFLND QVEIPTVEST KSG

2. Rna15p (polymer, Thiol state: all free), 109 monomers, 12040.69 Da Detail

GHMSNGPDFQ NSGNANFLSQ KFPELPSGID VNINMTTPAM MISSELAKKP KEVQLKFLQK FQEWTRAHPE DAASLLELCP QLSFVTAELL LTNGICKVDD LIPLASRPQ

Total weight

18089.516 Da

Max. entity weight

12040.69 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.4 %, Completeness: 97.6 %, Completeness (bb): 97.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.6 % (1872 of 1919)	97.6 % (980 of 1004)	97.6 % (725 of 743)	97.1 % (167 of 172)
Backbone	97.7 % (926 of 948)	97.2 % (309 of 318)	98.3 % (472 of 480)	96.7 % (145 of 150)
Sidechain	97.7 % (1101 of 1127)	97.8 % (671 of 686)	97.4 % (408 of 419)	100.0 % (22 of 22)
Aromatic	94.4 % (102 of 108)	94.4 % (51 of 54)	94.3 % (50 of 53)	100.0 % (1 of 1)
Methyl	100.0 % (188 of 188)	100.0 % (94 of 94)	100.0 % (94 of 94)

1. Rna14p

MKRDSELPTE VLDLLSVIPK RQYFNTNLLD AQKLVNFLND QVEIPTVEST KSG

2. Rna15p

GHMSNGPDFQ NSGNANFLSQ KFPELPSGID VNINMTTPAM MISSELAKKP KEVQLKFLQK FQEWTRAHPE DAASLLELCP QLSFVTAELL LTNGICKVDD LIPLASRPQ

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
1	Rna14p	[U-100% 13C; U-100% 15N; U-70% 2H]	1.0 mM
2	Rna15p	[U-100% 13C; U-100% 15N; U-70% 2H]	1.0 mM
3	sodium phosphate	natural abundance	20 mM
4	Nacl	natural abundance	50 mM
5	DTT	natural abundance	25 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
8	Rna14p	[U-99% 15N]	0.2-1.0 mM
9	Rna15p	[U-99% 15N]	0.2-1.0 mM
10	sodium phosphate	natural abundance	20 mM
11	Nacl	natural abundance	50 mM
12	DTT	natural abundance	25 mM
13	H2O	natural abundance	90 %
14	D2O	natural abundance	10 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
15	Rna14p	[U-99% 15N]	0.2 mM
16	Rna15p	[U-99% 15N]	0.2 mM
17	sodium phosphate	natural abundance	20 mM
18	Nacl	natural abundance	50 mM
19	DTT	natural abundance	25 mM
20	D2O	natural abundance	100 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
21	Rna14p	[U-99% 13C; U-99% 15N]	0.4 mM
22	Rna15p	[U-99% 13C; U-99% 15N]	0.4 mM
23	sodium phosphate	natural abundance	20 mM
24	Nacl	natural abundance	50 mM
25	DTT	natural abundance	25 mM
26	H2O	natural abundance	90 %
27	D2O	natural abundance	10 %

Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
28	Rna14p	[U-99% 13C; U-99% 15N]	0.4 mM
29	Rna15p	[U-99% 13C; U-99% 15N]	0.4 mM
30	sodium phosphate	natural abundance	20 mM
31	Nacl	natural abundance	50 mM
32	DTT	natural abundance	25 mM
33	D2O	natural abundance	100 %

Sample #6

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
34	Rna14p	[U-100% 13C; U-100% 15N; U-100% 2H, 1H-methyl Ile(delta 1),Leu,Val]	0.5 mM
35	Rna15p	[U-100% 13C; U-100% 15N; U-100% 2H, 1H-methyl Ile(delta 1),Leu,Val]	0.5 mM
36	sodium phosphate	natural abundance	20 mM
37	Nacl	natural abundance	50 mM
38	DTT	natural abundance	25 mM
39	H2O	natural abundance	90 %
40	D2O	natural abundance	10 %

Sample #7

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
41	Rna14p	[U-100% 13C; U-100% 15N; U-100% 2H, 1H-methyl Ile(delta 1),Leu,Val]	0.5 mM
42	Rna15p	[U-100% 13C; U-100% 15N; U-100% 2H, 1H-methyl Ile(delta 1),Leu,Val]	0.5 mM
43	sodium phosphate	natural abundance	20 mM
44	Nacl	natural abundance	50 mM
45	DTT	natural abundance	25 mM
46	D2O	natural abundance	100 %

Sample #8

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
47	Rna14p	[U-99% 15N];[U-99% 13C]-Leu	1.0 mM
48	Rna15p	[U-99% 15N];[U-99% 13C]-Leu	1.0 mM
49	sodium phosphate	natural abundance	20 mM
50	Nacl	natural abundance	50 mM
51	DTT	natural abundance	25 mM
52	H2O	natural abundance	90 %
53	D2O	natural abundance	10 %

Sample #9

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
54	Rna14p	[U-99% 15N];[U-99% 13C]-Leu	1.0 mM
55	Rna15p	[U-99% 15N];[U-99% 13C]-Leu	1.0 mM
56	sodium phosphate	natural abundance	20 mM
57	Nacl	natural abundance	50 mM
58	DTT	natural abundance	25 mM
59	D2O	natural abundance	100 %

Sample #10

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
60	Rna14p	[U-10% 13C; U-99% 15N]	1.0 mM
61	Rna15p	[U-10% 13C; U-99% 15N]	1.0 mM
62	sodium phosphate	natural abundance	20 mM
63	Nacl	natural abundance	50 mM
64	DTT	natural abundance	25 mM
65	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.725 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.725 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.725 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.725 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2L9B, Strand ID: A, B Detail

Release date

2010-10-25

Citation

Chemical shift assignments of a minimal Rna14p/Rna15p heterodimer from the yeast cleavage factor IA complex
Mackereth, C.D.
Biomol. NMR Assign. (2011), 5, 93-95, PubMed 20967574 , DOI 10.1007/s12104-010-9275-0 , Abstract

Related entities 1. Rna14p, : 1 : 2 : 3 entities Detail

Related entities 2. Rna15p, : 1 : 1 entities Detail

Interaction partners 1. Rna14p, : 21 interactors Detail

More details for 21 interactors identified by 6 citations

Experiments performed 30 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 700 MHz 5 mm PATXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
1	Rna14p	[U-100% 13C; U-100% 15N; U-70% 2H]	1.0 mM
2	Rna15p	[U-100% 13C; U-100% 15N; U-70% 2H]	1.0 mM
3	sodium phosphate	natural abundance	20 mM
4	Nacl	natural abundance	50 mM
5	DTT	natural abundance	25 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

2 3D HNCO

Instrument

Bruker Avance - 700 MHz 5 mm PATXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
1	Rna14p	[U-100% 13C; U-100% 15N; U-70% 2H]	1.0 mM
2	Rna15p	[U-100% 13C; U-100% 15N; U-70% 2H]	1.0 mM
3	sodium phosphate	natural abundance	20 mM
4	Nacl	natural abundance	50 mM
5	DTT	natural abundance	25 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

3 3D HN(CA)CO

Instrument

Bruker Avance - 700 MHz 5 mm PATXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
1	Rna14p	[U-100% 13C; U-100% 15N; U-70% 2H]	1.0 mM
2	Rna15p	[U-100% 13C; U-100% 15N; U-70% 2H]	1.0 mM
3	sodium phosphate	natural abundance	20 mM
4	Nacl	natural abundance	50 mM
5	DTT	natural abundance	25 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

4 3D HNCA

Instrument

Bruker Avance - 700 MHz 5 mm PATXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
1	Rna14p	[U-100% 13C; U-100% 15N; U-70% 2H]	1.0 mM
2	Rna15p	[U-100% 13C; U-100% 15N; U-70% 2H]	1.0 mM
3	sodium phosphate	natural abundance	20 mM
4	Nacl	natural abundance	50 mM
5	DTT	natural abundance	25 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

5 3D HN(CO)CA

Instrument

Bruker Avance - 700 MHz 5 mm PATXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
1	Rna14p	[U-100% 13C; U-100% 15N; U-70% 2H]	1.0 mM
2	Rna15p	[U-100% 13C; U-100% 15N; U-70% 2H]	1.0 mM
3	sodium phosphate	natural abundance	20 mM
4	Nacl	natural abundance	50 mM
5	DTT	natural abundance	25 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

6 3D HNCACB

Instrument

Bruker Avance - 700 MHz 5 mm PATXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
1	Rna14p	[U-100% 13C; U-100% 15N; U-70% 2H]	1.0 mM
2	Rna15p	[U-100% 13C; U-100% 15N; U-70% 2H]	1.0 mM
3	sodium phosphate	natural abundance	20 mM
4	Nacl	natural abundance	50 mM
5	DTT	natural abundance	25 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

7 3D CBCA(CO)NH

Instrument

Bruker Avance - 700 MHz 5 mm PATXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
1	Rna14p	[U-100% 13C; U-100% 15N; U-70% 2H]	1.0 mM
2	Rna15p	[U-100% 13C; U-100% 15N; U-70% 2H]	1.0 mM
3	sodium phosphate	natural abundance	20 mM
4	Nacl	natural abundance	50 mM
5	DTT	natural abundance	25 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

8 3D HNHA

Instrument

Bruker Avance - 700 MHz 5 mm PATXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
1	Rna14p	[U-100% 13C; U-100% 15N; U-70% 2H]	1.0 mM
2	Rna15p	[U-100% 13C; U-100% 15N; U-70% 2H]	1.0 mM
3	sodium phosphate	natural abundance	20 mM
4	Nacl	natural abundance	50 mM
5	DTT	natural abundance	25 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

9 3D C(CO)NH

Instrument

Bruker Avance - 700 MHz 5 mm PATXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
1	Rna14p	[U-100% 13C; U-100% 15N; U-70% 2H]	1.0 mM
2	Rna15p	[U-100% 13C; U-100% 15N; U-70% 2H]	1.0 mM
3	sodium phosphate	natural abundance	20 mM
4	Nacl	natural abundance	50 mM
5	DTT	natural abundance	25 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

10 3D H(CCO)NH

Instrument

Bruker Avance - 700 MHz 5 mm PATXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
1	Rna14p	[U-100% 13C; U-100% 15N; U-70% 2H]	1.0 mM
2	Rna15p	[U-100% 13C; U-100% 15N; U-70% 2H]	1.0 mM
3	sodium phosphate	natural abundance	20 mM
4	Nacl	natural abundance	50 mM
5	DTT	natural abundance	25 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

11 2D 1H-15N HSQC

Instrument

Bruker Avance - 700 MHz 5 mm PATXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
8	Rna14p	[U-99% 15N]	0.2-1.0 mM
9	Rna15p	[U-99% 15N]	0.2-1.0 mM
10	sodium phosphate	natural abundance	20 mM
11	Nacl	natural abundance	50 mM
12	DTT	natural abundance	25 mM
13	H2O	natural abundance	90 %
14	D2O	natural abundance	10 %

12 2D 1H-15N TROSY

Instrument

Bruker Avance - 700 MHz 5 mm PATXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
8	Rna14p	[U-99% 15N]	0.2-1.0 mM
9	Rna15p	[U-99% 15N]	0.2-1.0 mM
10	sodium phosphate	natural abundance	20 mM
11	Nacl	natural abundance	50 mM
12	DTT	natural abundance	25 mM
13	H2O	natural abundance	90 %
14	D2O	natural abundance	10 %

13 2D 1H-15N HMBC

Instrument

Bruker Avance - 700 MHz 5 mm PATXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
8	Rna14p	[U-99% 15N]	0.2-1.0 mM
9	Rna15p	[U-99% 15N]	0.2-1.0 mM
10	sodium phosphate	natural abundance	20 mM
11	Nacl	natural abundance	50 mM
12	DTT	natural abundance	25 mM
13	H2O	natural abundance	90 %
14	D2O	natural abundance	10 %

14 2D DQF-COSY

Instrument

Bruker Avance - 700 MHz 5 mm PATXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
15	Rna14p	[U-99% 15N]	0.2 mM
16	Rna15p	[U-99% 15N]	0.2 mM
17	sodium phosphate	natural abundance	20 mM
18	Nacl	natural abundance	50 mM
19	DTT	natural abundance	25 mM
20	D2O	natural abundance	100 %

15 3D H(CCO)NH

Instrument

Bruker Avance - 800 MHz 5 mm CPTXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
21	Rna14p	[U-99% 13C; U-99% 15N]	0.4 mM
22	Rna15p	[U-99% 13C; U-99% 15N]	0.4 mM
23	sodium phosphate	natural abundance	20 mM
24	Nacl	natural abundance	50 mM
25	DTT	natural abundance	25 mM
26	H2O	natural abundance	90 %
27	D2O	natural abundance	10 %

16 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz 5 mm CPTXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
28	Rna14p	[U-99% 13C; U-99% 15N]	0.4 mM
29	Rna15p	[U-99% 13C; U-99% 15N]	0.4 mM
30	sodium phosphate	natural abundance	20 mM
31	Nacl	natural abundance	50 mM
32	DTT	natural abundance	25 mM
33	D2O	natural abundance	100 %

17 2D HBHD

Instrument

Bruker Avance - 800 MHz 5 mm CPTXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
28	Rna14p	[U-99% 13C; U-99% 15N]	0.4 mM
29	Rna15p	[U-99% 13C; U-99% 15N]	0.4 mM
30	sodium phosphate	natural abundance	20 mM
31	Nacl	natural abundance	50 mM
32	DTT	natural abundance	25 mM
33	D2O	natural abundance	100 %

18 2D HBHE

Instrument

Bruker Avance - 800 MHz 5 mm CPTXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
28	Rna14p	[U-99% 13C; U-99% 15N]	0.4 mM
29	Rna15p	[U-99% 13C; U-99% 15N]	0.4 mM
30	sodium phosphate	natural abundance	20 mM
31	Nacl	natural abundance	50 mM
32	DTT	natural abundance	25 mM
33	D2O	natural abundance	100 %

19 3D HCACO

Instrument

Bruker Avance - 800 MHz 5 mm CPTXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
28	Rna14p	[U-99% 13C; U-99% 15N]	0.4 mM
29	Rna15p	[U-99% 13C; U-99% 15N]	0.4 mM
30	sodium phosphate	natural abundance	20 mM
31	Nacl	natural abundance	50 mM
32	DTT	natural abundance	25 mM
33	D2O	natural abundance	100 %

20 3D HC(C)H-TOCSY

Instrument

Bruker Avance - 800 MHz 5 mm CPTXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
28	Rna14p	[U-99% 13C; U-99% 15N]	0.4 mM
29	Rna15p	[U-99% 13C; U-99% 15N]	0.4 mM
30	sodium phosphate	natural abundance	20 mM
31	Nacl	natural abundance	50 mM
32	DTT	natural abundance	25 mM
33	D2O	natural abundance	100 %

21 3D (H)CCH-TOCSY

Instrument

Bruker Avance - 800 MHz 5 mm CPTXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
28	Rna14p	[U-99% 13C; U-99% 15N]	0.4 mM
29	Rna15p	[U-99% 13C; U-99% 15N]	0.4 mM
30	sodium phosphate	natural abundance	20 mM
31	Nacl	natural abundance	50 mM
32	DTT	natural abundance	25 mM
33	D2O	natural abundance	100 %

22 3D C(CO)NH

Instrument

Bruker Avance - 700 MHz 5 mm PATXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
34	Rna14p	[U-100% 13C; U-100% 15N; U-100% 2H, 1H-methyl Ile(delta 1),Leu,Val]	0.5 mM
35	Rna15p	[U-100% 13C; U-100% 15N; U-100% 2H, 1H-methyl Ile(delta 1),Leu,Val]	0.5 mM
36	sodium phosphate	natural abundance	20 mM
37	Nacl	natural abundance	50 mM
38	DTT	natural abundance	25 mM
39	H2O	natural abundance	90 %
40	D2O	natural abundance	10 %

23 2D 1H-13C HSQC

Instrument

Bruker Avance - 700 MHz 5 mm PATXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
41	Rna14p	[U-100% 13C; U-100% 15N; U-100% 2H, 1H-methyl Ile(delta 1),Leu,Val]	0.5 mM
42	Rna15p	[U-100% 13C; U-100% 15N; U-100% 2H, 1H-methyl Ile(delta 1),Leu,Val]	0.5 mM
43	sodium phosphate	natural abundance	20 mM
44	Nacl	natural abundance	50 mM
45	DTT	natural abundance	25 mM
46	D2O	natural abundance	100 %

24 3D HC(C)H-TOCSY

Instrument

Bruker Avance - 700 MHz 5 mm PATXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
41	Rna14p	[U-100% 13C; U-100% 15N; U-100% 2H, 1H-methyl Ile(delta 1),Leu,Val]	0.5 mM
42	Rna15p	[U-100% 13C; U-100% 15N; U-100% 2H, 1H-methyl Ile(delta 1),Leu,Val]	0.5 mM
43	sodium phosphate	natural abundance	20 mM
44	Nacl	natural abundance	50 mM
45	DTT	natural abundance	25 mM
46	D2O	natural abundance	100 %

25 3D (H)CCH-TOCSY

Instrument

Bruker Avance - 700 MHz 5 mm PATXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
41	Rna14p	[U-100% 13C; U-100% 15N; U-100% 2H, 1H-methyl Ile(delta 1),Leu,Val]	0.5 mM
42	Rna15p	[U-100% 13C; U-100% 15N; U-100% 2H, 1H-methyl Ile(delta 1),Leu,Val]	0.5 mM
43	sodium phosphate	natural abundance	20 mM
44	Nacl	natural abundance	50 mM
45	DTT	natural abundance	25 mM
46	D2O	natural abundance	100 %

26 2D HNCO

Instrument

Bruker Avance - 700 MHz 5 mm PATXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
47	Rna14p	[U-99% 15N];[U-99% 13C]-Leu	1.0 mM
48	Rna15p	[U-99% 15N];[U-99% 13C]-Leu	1.0 mM
49	sodium phosphate	natural abundance	20 mM
50	Nacl	natural abundance	50 mM
51	DTT	natural abundance	25 mM
52	H2O	natural abundance	90 %
53	D2O	natural abundance	10 %

27 3D HCACO

Instrument

Bruker Avance - 700 MHz 5 mm PATXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
54	Rna14p	[U-99% 15N];[U-99% 13C]-Leu	1.0 mM
55	Rna15p	[U-99% 15N];[U-99% 13C]-Leu	1.0 mM
56	sodium phosphate	natural abundance	20 mM
57	Nacl	natural abundance	50 mM
58	DTT	natural abundance	25 mM
59	D2O	natural abundance	100 %

28 3D (H)CCH-TOCSY

Instrument

Bruker Avance - 700 MHz 5 mm PATXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
54	Rna14p	[U-99% 15N];[U-99% 13C]-Leu	1.0 mM
55	Rna15p	[U-99% 15N];[U-99% 13C]-Leu	1.0 mM
56	sodium phosphate	natural abundance	20 mM
57	Nacl	natural abundance	50 mM
58	DTT	natural abundance	25 mM
59	D2O	natural abundance	100 %

29 3D HC(C)H-TOCSY

Instrument

Bruker Avance - 700 MHz 5 mm PATXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
54	Rna14p	[U-99% 15N];[U-99% 13C]-Leu	1.0 mM
55	Rna15p	[U-99% 15N];[U-99% 13C]-Leu	1.0 mM
56	sodium phosphate	natural abundance	20 mM
57	Nacl	natural abundance	50 mM
58	DTT	natural abundance	25 mM
59	D2O	natural abundance	100 %

30 2D 1H-13C HSQC-CT

Instrument

Bruker Avance - 700 MHz 5 mm PATXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 20 mM sodium phosphate, 50 mM NaCl, 2 mM DTT, pH 6.5

#	Name	Isotope labeling	Concentration
60	Rna14p	[U-10% 13C; U-99% 15N]	1.0 mM
61	Rna15p	[U-10% 13C; U-99% 15N]	1.0 mM
62	sodium phosphate	natural abundance	20 mM
63	Nacl	natural abundance	50 mM
64	DTT	natural abundance	25 mM
65	D2O	natural abundance	100 %

null

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Found 1 Document (1.362 seconds)

BMRB: 17161

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Rna14p, : 1 : 2 : 3 entities Detail

Related entities 2. Rna15p, : 1 : 1 entities Detail

Interaction partners 1. Rna14p, : 21 interactors Detail

Experiments performed 30 experiments Detail

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