Prion-like conversion during amyloid formation at atomic resolution
MIQVYSRHPA ENGKSNFLNC YVSGFHPSDI EVDLLKNGER IEKVEHSDLS FSKDWSFYLL YYTEFTPTEK DEYACRVNHV TLSQPKIVKW DRDM
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 94.7 % (1081 of 1141) | 95.1 % (565 of 594) | 94.4 % (423 of 448) | 93.9 % (93 of 99) |
Backbone | 96.4 % (536 of 556) | 96.8 % (181 of 187) | 96.4 % (269 of 279) | 95.6 % (86 of 90) |
Sidechain | 93.6 % (633 of 676) | 94.3 % (384 of 407) | 93.1 % (242 of 260) | 77.8 % (7 of 9) |
Aromatic | 84.1 % (116 of 138) | 87.0 % (60 of 69) | 80.6 % (54 of 67) | 100.0 % (2 of 2) |
Methyl | 100.0 % (84 of 84) | 100.0 % (42 of 42) | 100.0 % (42 of 42) |
1. dN6
MIQVYSRHPA ENGKSNFLNC YVSGFHPSDI EVDLLKNGER IEKVEHSDLS FSKDWSFYLL YYTEFTPTEK DEYACRVNHV TLSQPKIVKW DRDMSolvent system 90% H2O/10% D2O, Pressure 1.000 (±0.100) atm, Temperature 298.000 (±1.000) K, pH 7.500 (±0.100), Details backbone assignment, 15N_noesy
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | dN6 | [U-100% 13C; U-100% 15N] | 0.5 (±5.0E-5) mM | |
2 | sodium phosphate | natural abundance | 25.0 (±0.0025) mM | |
3 | sodium acide | natural abundance | 1.0 (±1.0E-4) mM | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Pressure 1.000 (±0.100) atm, Temperature 298.000 (±1.000) K, pH 7.500 (±0.100), Details side chain assignment, 13C_noesy
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | dN6 | [U-100% 13C; U-100% 15N] | 1.0 (±1.0E-4) mM | |
7 | sodium phosphate | natural abundance | 25.0 (±0.0025) mM | |
8 | sodium acide | natural abundance | 1.0 (±1.0E-4) mM | |
9 | D2O | natural abundance | 100 % |