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BMRB: 17173

2LO6

1H, 13C, and 15N chemical shift assignments of the Nrd1-CTD Interacting Domain

Authors

Kubicek, K., Pasulka, J., Cerna, H., Loehr, F., Stefl, R.

Assembly

Nrd1 CID

Entity

1. Nrd1 CID (polymer, Thiol state: all free), 161 monomers, 18286.42 Da Detail

MQQDDDFQNF VATLESFKDL KSGISGSRIK KLTTYALDHI DIESKIISLI IDYSRLCPDS HKLGSLYIID SIGRAYLDET RSNSNSSSNK PGTCAHAINT LGEVIQELLS DAIAKSNQDH KEKIRMLLDI WDRSGLFQKS YLNAIRSKCF AMDLEHHHHH H

Formula weight

18286.42 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 78.3 %, Completeness: 57.6 %, Completeness (bb): 63.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	57.6 % (1083 of 1881)	60.2 % (588 of 977)	51.2 % (374 of 731)	69.9 % (121 of 173)
Backbone	63.7 % (613 of 962)	74.0 % (242 of 327)	53.2 % (253 of 476)	74.2 % (118 of 159)
Sidechain	54.8 % (588 of 1073)	53.2 % (346 of 650)	58.4 % (239 of 409)	21.4 % (3 of 14)
Aromatic	19.0 % (27 of 142)	22.5 % (16 of 71)	14.3 % (10 of 70)	100.0 % (1 of 1)
Methyl	82.8 % (144 of 174)	82.8 % (72 of 87)	82.8 % (72 of 87)

1. Nrd1 CID

MQQDDDFQNF VATLESFKDL KSGISGSRIK KLTTYALDHI DIESKIISLI IDYSRLCPDS HKLGSLYIID SIGRAYLDET RSNSNSSSNK PGTCAHAINT LGEVIQELLS DAIAKSNQDH KEKIRMLLDI WDRSGLFQKS YLNAIRSKCF AMDLEHHHHH H

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 8.0, Details 50 mM Na2HPO4, 100mM NaCl, 10mM β mercaptoethanol, pH=8,0

#	Name	Isotope labeling	Concentration
1	Nrd1 CID	[U-100% 13C; U-100% 15N]	2 mM
2	Na2HPO4	natural abundance	50 mM
3	NaCl	natural abundance	100 mM
4	b-mercaptoethanol	natural abundance	10 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 2.29 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 2.29 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.17 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LO6, Strand ID: A Detail

Release date

2011-03-29

Citation

1H, 13C, and 15N resonance assignments for the CTD-interacting domain of Nrd1 bound to Ser5-phosphorylated CTD of RNA polymerase II
Kubiek, K., Pasulka, J., erna, H., Lohr, F., tefl, R.
Biomol. NMR Assign. (2011), 5, 203-205, PubMed 21350922 , DOI 10.1007/s12104-011-9300-y , Abstract

Related entities 1. Nrd1 CID, : 1 : 2 : 1 : 41 entities Detail

Interaction partners 1. Nrd1 CID, : 28 interactors Detail

More details for 28 interactors identified by 6 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC