Solution NMR structure of hepothetical homodimer protein SP_0782 (7-79) from Streptococcus pneumoniae, Northeast Structural Genomics Consortium Target SpR104.
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 93.1 % (920 of 988) | 93.2 % (480 of 515) | 91.7 % (353 of 385) | 98.9 % (87 of 88) |
Backbone | 99.0 % (483 of 488) | 99.4 % (166 of 167) | 98.8 % (238 of 241) | 98.8 % (79 of 80) |
Sidechain | 89.1 % (514 of 577) | 90.2 % (314 of 348) | 86.9 % (192 of 221) | 100.0 % (8 of 8) |
Aromatic | 62.1 % (72 of 116) | 63.8 % (37 of 58) | 58.9 % (33 of 56) | 100.0 % (2 of 2) |
Methyl | 100.0 % (66 of 66) | 100.0 % (33 of 33) | 100.0 % (33 of 33) |
1. SP 0782
MKKMAEFTFE IEEHLLTLSE NEKGWTKEIN RVSFNGAPAK FDIRAWSPDH TKMGKGITLS NEEFQTMVDA FKGNLEHHHH HHSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 4.5 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | homodimer protein SP_0782 (7-79) from Streptococcus pneumoniae | [U-100% 13C; U-100% 15N] | 1.2 (±0.12) mM | |
2 | ammonium acetate | natural abundance | 20 (±1.0) mM | |
3 | sodium chloride | natural abundance | 100 (±5.0) mM | |
4 | calcium chloride | natural abundance | 5 (±0.2) mM | |
5 | DTT | natural abundance | 10 (±0.5) mM | |
6 | sodium azide | natural abundance | 0.02 (±0.001) % | |
7 | H2O | natural abundance | 90 % | |
8 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 4.5 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | homodimer protein SP_0782 (7-79) from Streptococcus pneumoniae | [U-5% 13C; U-100% 15N] | 1.2 (±0.12) mM | |
10 | ammonium acetate | natural abundance | 20 (±1.0) mM | |
11 | sodium chloride | natural abundance | 100 (±5.0) mM | |
12 | calcium chloride | natural abundance | 5 (±0.2) mM | |
13 | DTT | natural abundance | 10 (±0.5) mM | |
14 | sodium azide | natural abundance | 0.02 (±0.001) % | |
15 | H2O | natural abundance | 90 % | |
16 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 4.5 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
17 | homodimer protein SP_0782 (7-79) from Streptococcus pneumoniae | [U-100% 13C; U-100% 15N] | 1.2 (±0.12) mM | |
18 | ammonium acetate | natural abundance | 20 (±1.0) mM | |
19 | sodium chloride | natural abundance | 100 (±5.0) mM | |
20 | calcium chloride | natural abundance | 5 (±0.2) mM | |
21 | DTT | natural abundance | 10 (±0.5) mM | |
22 | sodium azide | natural abundance | 0.02 (±0.001) % | |
23 | D2O | natural abundance | 100 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 4.5 (±0.1), Details mixted protein sample of 1:1 concentration ratio of NC sample:natural abundance sample. (both 1.2mM before being mixed together)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
24 | homodimer protein SP_0782 (7-79) from Streptococcus pneumoniae | [U-100% 13C; U-100% 15N] | 1.2 (±0.12) mM | |
25 | homodimer protein SP_0782 (7-79) from Streptococcus pneumoniae | natural abundance | 1.2 (±0.12) mM | |
26 | ammonium acetate | natural abundance | 20 (±1.0) mM | |
27 | sodium chloride | natural abundance | 100 (±5.0) mM | |
28 | calcium chloride | natural abundance | 5 (±0.2) mM | |
29 | DTT | natural abundance | 10 (±0.5) mM | |
30 | sodium azide | natural abundance | 0.02 (±0.001) % | |
31 | H2O | natural abundance | 90 % | |
32 | D2O | natural abundance | 10 % |