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BMRB: 17204

2L32

the 1H, 13C and 15N resonance assignment of ubiquitin-like small archaeal modifier protein from Haloferax volcanii

Authors

Zhang, W., Fan, K., Zhang, J., Tu, X.

Assembly

ubiquitin-like small archaeal modifier protein

Entity

1. ubiquitin-like small archaeal modifier protein (polymer, Thiol state: unknown), 74 monomers, 8182.965 Da Detail

MNVTVEVVGE ETSEVAVDDD GTYADLVRAV DLSPHEVTVL VDGRPVPEDQ SVEVDRVKVL RLIKGGLEHH HHHH

Formula weight

8182.965 Da

Source organism

Haloferax volcanii

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.5 %, Completeness: 67.1 %, Completeness (bb): 70.5 % Detail

Polymer type: polypeptide(D)

	Total	¹H	¹³C	¹⁵N
All	67.1 % (540 of 805)	70.9 % (290 of 409)	58.2 % (188 of 323)	84.9 % (62 of 73)
Backbone	70.5 % (309 of 438)	80.0 % (120 of 150)	58.5 % (127 of 217)	87.3 % (62 of 71)
Sidechain	66.1 % (288 of 436)	65.6 % (170 of 259)	67.4 % (118 of 175)	0.0 % (0 of 2)
Aromatic	0.0 % (0 of 36)	0.0 % (0 of 18)	0.0 % (0 of 18)
Methyl	79.2 % (84 of 106)	83.0 % (44 of 53)	75.5 % (40 of 53)

1. ubiquitin-like small archaeal modifier protein

MNVTVEVVGE ETSEVAVDDD GTYADLVRAV DLSPHEVTVL VDGRPVPEDQ SVEVDRVKVL RLIKGGLEHH HHHH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
1	submit 2	[U-100% 13C; U-100% 15N]		1 mM
2	sodium phosphate	natural abundance		25 mM
3	sodium chloride	natural abundance		100 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

LACS Plot; CA

Referencing offset: 0.12 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: 0.12 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Release date

2010-09-23

Citation

Ionic strength-dependent conformations of a ubiquitin-like small archaeal modifier protein (SAMP2) from Haloferax volcanii
Zhang, W., Liao, S., Fan, K., Ye, K., Zhang, X., Zhang, J., Xu, C., Tu, X.
Sci. Rep. (2013), 3, 2136-2136, PubMed 23823798 , DOI 10.1038/srep02136 , Abstract

Related entities 1. ubiquitin-like small archaeal modifier protein, : 1 : 1 : 2 : 1 entities Detail

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
1	submit 2	[U-100% 13C; U-100% 15N]		1 mM
2	sodium phosphate	natural abundance		25 mM
3	sodium chloride	natural abundance		100 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

2 3D HBHA(CO)NH

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
1	submit 2	[U-100% 13C; U-100% 15N]		1 mM
2	sodium phosphate	natural abundance		25 mM
3	sodium chloride	natural abundance		100 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

3 3D H(CCO)NH

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
1	submit 2	[U-100% 13C; U-100% 15N]		1 mM
2	sodium phosphate	natural abundance		25 mM
3	sodium chloride	natural abundance		100 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

4 3D CBCA(CO)NH

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
1	submit 2	[U-100% 13C; U-100% 15N]		1 mM
2	sodium phosphate	natural abundance		25 mM
3	sodium chloride	natural abundance		100 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

5 3D C(CO)NH

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
1	submit 2	[U-100% 13C; U-100% 15N]		1 mM
2	sodium phosphate	natural abundance		25 mM
3	sodium chloride	natural abundance		100 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

6 3D HNCACB

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
1	submit 2	[U-100% 13C; U-100% 15N]		1 mM
2	sodium phosphate	natural abundance		25 mM
3	sodium chloride	natural abundance		100 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

7 3D 1H-15N NOESY

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
1	submit 2	[U-100% 13C; U-100% 15N]		1 mM
2	sodium phosphate	natural abundance		25 mM
3	sodium chloride	natural abundance		100 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr17204_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr17204_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	607		89.2 (chain: 1, length: 74)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 74, Sequence coverage: 89.2%
- Total number of assignments
  - ¹H chemical shifts: 367
  - ¹³C chemical shifts: 179
  - ¹⁵N chemical shifts: 61
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1