BMRBj - BMREntryMaster

Found 1 Document (2.788 seconds)

BMRB: 17208

7KZI

Backbone 1H, 13C, and 15N chemical shift assignments for the nucleotide-binding domain of E.coli DnaK in the ADP.Pi-bound state

Authors

Zhuravleva, A., Gierasch, L.M.

Assembly

NBD(1-388)

Entity

1. the nucleotide-binding domain of DnaK (polymer, Thiol state: all free), 388 monomers, 42009.22 Da Detail

MGKIIGIDLG TTNSCVAIMD GTTPRVLENA EGDRTTPSII AYTQDGETLV GQPAKRQAVT NPQNTLFAIK RLIGRRFQDE EVQRDVSIMP FKIIAADNGD AWVEVKGQKM APPQISAEVL KKMKKTAEDY LGEPVTEAVI TVPAYFNDAQ RQATKDAGRI AGLEVKRIIN EPTAAALAYG LDKGTGNRTI AVYDLGGGAF DISIIEIDEV DGEKTFEVLA TNGDTHLGGE DFDSRLINYL VEEFKKDQGI DLRNDPLAMQ RLKEAAEKAK IELSSAQQTD VNLPYITADA TGPKHMNIKV TRAKLESLVE DLVNRSIELL KVALQDAGLS VSDIDDVILV GGQTRMPMVQ KKVAEFFGKE PRKDVNPDEA VAIGAAVQGG VLTGDVKD

2. ADP (non-polymer), 427.201 Da
3. MG (non-polymer), 24.305 Da
4. PO4 (non-polymer), 94.971 Da

Total weight

42555.695 Da

Max. entity weight

42009.22 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.4 %, Completeness: 45.7 %, Completeness (bb): 77.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	45.7 % (1998 of 4374)	24.4 % (553 of 2267)	64.9 % (1104 of 1701)	84.0 % (341 of 406)
Backbone	77.4 % (1777 of 2296)	50.4 % (400 of 793)	91.7 % (1037 of 1131)	91.4 % (340 of 372)
Sidechain	21.9 % (533 of 2433)	10.4 % (153 of 1474)	41.0 % (379 of 925)	2.9 % (1 of 34)
Aromatic	10.8 % (19 of 176)	14.8 % (13 of 88)	6.9 % (6 of 87)	0.0 % (0 of 1)
Methyl	21.8 % (108 of 496)	15.7 % (39 of 248)	27.8 % (69 of 248)

1. the nucleotide-binding domain of DnaK

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 299 (±0.2) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	potassium phosphate	natural abundance	10 mM
2	magnesium chloride	natural abundance	10 mM
3	AEBSF protease inhibitor	natural abundance	1 uM
4	DTT	natural abundance	5 mM
5	DSS	natural abundance	0.5 mM
6	ADP	natural abundance	10 mM
7	potassium chloride	natural abundance	10 mM
8	the nucleotide-binding domain of DnaK	[U-97% 2H; U-95% 13C; U-95% 15N]	0.5 mM
9	H2O	natural abundance	95 %
10	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.11 ppm, Outliers: 2 Detail

Release date

2011-05-04

Citation 1

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , Abstract

Show more 3 citaions

Citation 2

Allosteric signal transmission in the nucleotide-binding domain of 70-kDa heat shock protein (Hsp70) molecular chaperones
Zhuravleva, A., Gierasch, L.M.
Proc. Natl. Acad. Sci. U. S. A. (2011), 108, 6987-6992, PubMed 21482798 , DOI 10.1073/pnas.1014448108 , Abstract

Citation 3

Automated analysis of protein NMR assignments using methods from artificial intelligence
Feng, W., Zimmerman, D., Kulikowski, C., Huang, Y., Tashiro, M., Shimotakahara, S., Chien, C., Powers, R., Montelione, G.
J. Mol. Biol. (1997), 269, 592-610, PubMed 9217263 , DOI 10.1006/jmbi.1997.1052 , Abstract

Citation 4

Optimizing the process of nuclear magnetic resonance spectrum analysis and computer aided resonance assignment
Keller, R.

Hide non-primary 3 citaions

Related entities 1. the nucleotide-binding domain of DnaK, : 1 : 18 : 283 entities Detail

Interaction partners 1. the nucleotide-binding domain of DnaK, : 322 interactors Detail

More details for 322 interactors identified by 9 citations

Experiments performed 6 experiments Detail

1 2D 1H-15N TROSY