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BMRB: 17212

2L42

1H, 13C and 15N resonance assignment of Rap1 BRCT domain from Saccharomyces cerevisiae

Authors

Zhang, W., Zhang, J., Tu, X.

Assembly

Rap1 BRCT domain

Entity

1. Rap1 BRCT domain (polymer, Thiol state: unknown), 106 monomers, 12167.45 Da Detail

MEKKEQVSGP PLSNMKFYLN RDADAHDSLN DIDQLARLIR ANGGEVLDSK PRESKENVFI VSPYNHTNLP TVTPTYIKAC CQSNSLLNME NYLVPYDNLE HHHHHH

Formula weight

12167.45 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.5 %, Completeness: 70.9 %, Completeness (bb): 87.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	70.9 % (878 of 1238)	67.2 % (435 of 647)	73.8 % (353 of 478)	79.6 % (90 of 113)
Backbone	87.1 % (542 of 622)	85.1 % (177 of 208)	87.3 % (275 of 315)	90.9 % (90 of 99)
Sidechain	59.7 % (429 of 719)	59.0 % (259 of 439)	63.9 % (170 of 266)	0.0 % (0 of 14)
Aromatic	13.0 % (12 of 92)	13.0 % (6 of 46)	13.0 % (6 of 46)
Methyl	80.4 % (82 of 102)	78.4 % (40 of 51)	82.4 % (42 of 51)

1. Rap1 BRCT domain

MEKKEQVSGP PLSNMKFYLN RDADAHDSLN DIDQLARLIR ANGGEVLDSK PRESKENVFI VSPYNHTNLP TVTPTYIKAC CQSNSLLNME NYLVPYDNLE HHHHHH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	20 mM
2	sodium chloride	natural abundance	100 mM
3	Rap1 BRCT domain	natural abundance	0.8 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291
³¹P	DSS	methyl protons	na	indirect	0.4048086

Referencing offset: 0.04 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291
³¹P	DSS	methyl protons	na	indirect	0.4048086

Referencing offset: 0.04 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291
³¹P	DSS	methyl protons	na	indirect	0.4048086

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291
³¹P	DSS	methyl protons	na	indirect	0.4048086

Referencing offset: 0.16 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L42, Strand ID: A Detail

Release date

2011-03-29

Citation

Solution structure of Rap1 BRCT domain from Saccharomyces cerevisiae reveals a novel fold
Zhang, W., Zhang, J., Zhang, X., Xu, C., Tu, X.
Biochem. Biophys. Res. Commun. (2011), 404, 1055-1059, PubMed 21187076 , DOI 10.1016/j.bbrc.2010.12.109 , Abstract

Related entities 1. Rap1 BRCT domain, : 1 : 2 entities Detail

Interaction partners 1. Rap1 BRCT domain, : 33 interactors Detail

More details for 33 interactors identified by 11 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC