1H, 13C and 15N resonance assignment of Rap1 BRCT domain from Saccharomyces cerevisiae
MEKKEQVSGP PLSNMKFYLN RDADAHDSLN DIDQLARLIR ANGGEVLDSK PRESKENVFI VSPYNHTNLP TVTPTYIKAC CQSNSLLNME NYLVPYDNLE HHHHHH
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 70.9 % (878 of 1238) | 67.2 % (435 of 647) | 73.8 % (353 of 478) | 79.6 % (90 of 113) |
Backbone | 87.1 % (542 of 622) | 85.1 % (177 of 208) | 87.3 % (275 of 315) | 90.9 % (90 of 99) |
Sidechain | 59.7 % (429 of 719) | 59.0 % (259 of 439) | 63.9 % (170 of 266) | 0.0 % (0 of 14) |
Aromatic | 13.0 % (12 of 92) | 13.0 % (6 of 46) | 13.0 % (6 of 46) | |
Methyl | 80.4 % (82 of 102) | 78.4 % (40 of 51) | 82.4 % (42 of 51) |
1. Rap1 BRCT domain
MEKKEQVSGP PLSNMKFYLN RDADAHDSLN DIDQLARLIR ANGGEVLDSK PRESKENVFI VSPYNHTNLP TVTPTYIKAC CQSNSLLNME NYLVPYDNLE HHHHHHSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | sodium phosphate | natural abundance | 20 mM | |
2 | sodium chloride | natural abundance | 100 mM | |
3 | Rap1 BRCT domain | natural abundance | 0.8 mM |