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BMRB: 17254

2L4S

Promiscuous Binding at the Crossroads of Numerous Cancer Pathways: Insight from the Binding of GIP with Glutaminase L

Authors

Zoetewey, D.L., Ovee, M., Banerjee, M., Bhaskaran, R., Mohanty, S.

Assembly

GIP apo

Entity

1. GIP apo (polymer, Thiol state: not present), 124 monomers, 13734.54 Da Detail

MSYIPGQPVT AVVQRVEIHK LRQGENLILG FSIGGGIDQD PSQNPFSEDK TDKGIYVTRV SEGGPAEIAG LQIGDKIMQV NGWDMTMVTH DQARKRLTKR SEEVVRLLVT RQSLQKAVQQ SMLS

Formula weight

13734.54 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 93.9 %, Completeness (bb): 98.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.9 % (1349 of 1436)	96.0 % (723 of 753)	90.0 % (493 of 548)	98.5 % (133 of 135)
Backbone	98.4 % (722 of 734)	98.0 % (250 of 255)	98.6 % (355 of 360)	98.3 % (117 of 119)
Sidechain	90.5 % (737 of 814)	95.0 % (473 of 498)	82.7 % (248 of 300)	100.0 % (16 of 16)
Aromatic	71.4 % (40 of 56)	82.1 % (23 of 28)	59.3 % (16 of 27)	100.0 % (1 of 1)
Methyl	86.1 % (124 of 144)	98.6 % (71 of 72)	73.6 % (53 of 72)

1. Glutaminase Interacting Protein 3

MSYIPGQPVT AVVQRVEIHK LRQGENLILG FSIGGGIDQD PSQNPFSEDK TDKGIYVTRV SEGGPAEIAG LQIGDKIMQV NGWDMTMVTH DQARKRLTKR SEEVVRLLVT RQSLQKAVQQ SMLS

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Glutaminase Interacting Protein 3	[U-99% 13C; U-99% 15N]	0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium azide	natural abundance	0.1 % w/v
4	EDTA	natural abundance	1 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.73 ppm	internal	indirect	0.2514495
¹H	water	protons	4.73 ppm	internal	direct	1.0
¹⁵N	water	protons	4.73 ppm	internal	indirect	0.1013291

Referencing offset: 0.11 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.73 ppm	internal	indirect	0.2514495
¹H	water	protons	4.73 ppm	internal	direct	1.0
¹⁵N	water	protons	4.73 ppm	internal	indirect	0.1013291

Referencing offset: 0.11 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.73 ppm	internal	indirect	0.2514495
¹H	water	protons	4.73 ppm	internal	direct	1.0
¹⁵N	water	protons	4.73 ppm	internal	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.73 ppm	internal	indirect	0.2514495
¹H	water	protons	4.73 ppm	internal	direct	1.0
¹⁵N	water	protons	4.73 ppm	internal	indirect	0.1013291

Referencing offset: 0.34 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L4S, Strand ID: A Detail

Release date

2010-10-12

Citation

Promiscuous binding at the crossroads of numerous cancer pathways: insight from the binding of glutaminase interacting protein with glutaminase L
Zoetewey, D.L., Ovee, M., Banerjee, M., Bhaskaran, R., Mohanty, S.
Biochemistry (2011), 50, 3528-3539, PubMed 21417405 , DOI 10.1021/bi102055y , Abstract

Related entities 1. GIP apo, : 1 : 4 : 10 : 91 entities Detail

Interaction partners 1. GIP apo, : 16 interactors Detail

More details for 16 interactors identified by 6 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC