NMR structure of the Xanthomonas VirB7
GSHMTKPAPD FGGRWKHVNH FDEAPTEIPL YTSYTYQATP MDGTLKTMLE RWAADSNMQL SYNLPSDYTL IGPVSAISTT SVQQAATELS AVYAAQGVSV SVSANKLLVQ PVPVSSGAKL
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 97.1 % (1292 of 1331) | 97.1 % (660 of 680) | 97.0 % (512 of 528) | 97.6 % (120 of 123) |
Backbone | 96.9 % (680 of 702) | 97.1 % (231 of 238) | 96.9 % (342 of 353) | 96.4 % (107 of 111) |
Sidechain | 97.2 % (721 of 742) | 96.8 % (428 of 442) | 97.6 % (281 of 288) | 100.0 % (12 of 12) |
Aromatic | 94.2 % (98 of 104) | 94.2 % (49 of 52) | 94.0 % (47 of 50) | 100.0 % (2 of 2) |
Methyl | 98.6 % (138 of 140) | 98.6 % (69 of 70) | 98.6 % (69 of 70) |
1. VirB7-Xac2622
GSHMTKPAPD FGGRWKHVNH FDEAPTEIPL YTSYTYQATP MDGTLKTMLE RWAADSNMQL SYNLPSDYTL IGPVSAISTT SVQQAATELS AVYAAQGVSV SVSANKLLVQ PVPVSSGAKLSolvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0, Details 10mM deuterated sodium acetate buffer pH 5.0 50mM NaCl
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | VirB7 - Xac2622 | [U-99% 13C; U-99% 15N] | 0.2 mM | |
2 | sodium acetate | [U-99% 2H] | 10 mM | |
3 | sodium chloride | natural abundance | 50 mM | |
4 | H2O | natural abundance | 93 % | |
5 | D2O | natural abundance | 7 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0, Details 10mM deuterated sodium acetate buffer pH 5.0 50mM NaCl
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | VirB7 - Xac2622 | [U-99% 13C; U-99% 15N] | 0.2 mM | |
7 | sodium acetate | [U-99% 2H] | 10 mM | |
8 | sodium chloride | natural abundance | 50 mM | |
9 | D2O | natural abundance | 100 % |