Resonance assignments and secondary structure of a phytocystatin from Ananas comosus
MADEQPKKPV PAAEEAPAAE AEAEEEEGLH LEDDQEPREH PIMGGIYDAP LNNENGFDKE DLARFAVREY NNKNNALLEF VRVVKAKEQV VSGMMHYLTV EVNDAGKKKL YEAKVWEQVW MNFRQLQEFT YLGDA
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 95.1 % (1509 of 1587) | 98.1 % (816 of 832) | 90.0 % (549 of 610) | 99.3 % (144 of 145) |
Backbone | 99.5 % (792 of 796) | 98.9 % (267 of 270) | 100.0 % (398 of 398) | 99.2 % (127 of 128) |
Sidechain | 91.9 % (845 of 919) | 97.7 % (549 of 562) | 82.1 % (279 of 340) | 100.0 % (17 of 17) |
Aromatic | 46.8 % (59 of 126) | 90.5 % (57 of 63) | 0.0 % (0 of 61) | 100.0 % (2 of 2) |
Methyl | 99.2 % (127 of 128) | 98.4 % (63 of 64) | 100.0 % (64 of 64) |
1. Pineapple Cystatin
MADEQPKKPV PAAEEAPAAE AEAEEEEGLH LEDDQEPREH PIMGGIYDAP LNNENGFDKE DLARFAVREY NNKNNALLEF VRVVKAKEQV VSGMMHYLTV EVNDAGKKKL YEAKVWEQVW MNFRQLQEFT YLGDASolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.5 (±0.05), Details in 20mM KCl, 1mM NaN3, 1mM 2-mercaptoethanol, 4mM Benzamidine
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Pineapple Cystatin | [U-99% 13C; U-99% 15N] | 0.7 ~ 1.0 (±0.05) mM | |
2 | KCl | natural abundance | 20 mM | |
3 | NaN3 | natural abundance | 1 mM | |
4 | 2-mercaptoethanol | natural abundance | 1 mM | |
5 | Benzamidine | natural abundance | 4 mM | |
6 | H2O | natural abundance | 90 % | |
7 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.5 (±0.05), Details in 20mM KCl, 1mM NaN3, 1mM 2-mercaptoethanol, 4mM Benzamidine
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | Pineapple Cystatin | [U-99% 13C; U-99% 15N] | 0.7 ~ 1.0 (±0.05) mM | |
9 | KCl | natural abundance | 20 mM | |
10 | NaN3 | natural abundance | 1 mM | |
11 | 2-mercaptoethanol | natural abundance | 1 mM | |
12 | Benzamidine | natural abundance | 4 mM | |
13 | D2O | natural abundance | 100 % |