Backbone and side-chain 1H, 13C and 15N assignments of the UCHL1 S18Y variant
MQLKPMEINP EMLNKVLYRL GVAGQWRFVD VLGLEEESLG SVPAPACALL LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA NNQDKLGFED GSVLKQFLSE TEKMSPEDRA KCFEKNEAIQ AAHDAVAQEG QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DTLLKDAAKV CREFTEREQG EVRFSAVALC KAALEHHHHH H
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 83.3 % (2251 of 2702) | 81.7 % (1151 of 1408) | 82.4 % (864 of 1048) | 95.9 % (236 of 246) |
Backbone | 96.8 % (1324 of 1368) | 94.9 % (444 of 468) | 97.8 % (663 of 678) | 97.7 % (217 of 222) |
Sidechain | 73.3 % (1136 of 1550) | 75.3 % (708 of 940) | 69.8 % (409 of 586) | 79.2 % (19 of 24) |
Aromatic | 6.5 % (14 of 214) | 7.5 % (8 of 107) | 4.7 % (5 of 106) | 100.0 % (1 of 1) |
Methyl | 91.4 % (223 of 244) | 94.3 % (115 of 122) | 88.5 % (108 of 122) |
1. UCHL1 S18Y variant
MQLKPMEINP EMLNKVLYRL GVAGQWRFVD VLGLEEESLG SVPAPACALL LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA NNQDKLGFED GSVLKQFLSE TEKMSPEDRA KCFEKNEAIQ AAHDAVAQEG QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DTLLKDAAKV CREFTEREQG EVRFSAVALC KAALEHHHHH HSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | UCHL1 S18Y variant | [U-99% 13C; U-99% 15N] | 0.7 ~ 0.8 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 100 mM | |
4 | DTT | natural abundance | 3 mM | |
5 | H2O | natural abundance | 90 % | |
6 | D2O | natural abundance | 10 % |