BMRBj - BMREntryMaster

Found 1 Document (0.729 seconds)

BMRB: 17266

2L55

Solution structure of the C-terminal domain of SilB from Cupriavidus metallidurans

Authors

Bersch, B., Derfoufi, K., Vandenbussche, G.

Assembly

SilB(440-521)

Entity

1. SilB(440-521) (polymer, Thiol state: not present), 82 monomers, 8540.724 Da Detail

GPEHRAVGRI QSIGERSLII AHEAIPSAQW GAMTMEFAAP PAGLPQGLKA GDRVAFSFRL DPHGMATLVT VAPQVQTAGA KP

Formula weight

8540.724 Da

Source organism

Cupriavidus metallidurans

Exptl. method

solution NMR

Refine. method

simulated annealing, simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts, heteronucl_NOEs, heteronucl_T1_relaxation, heteronucl_T2_relaxation

Chem. Shift Complete

Sequence coverage: 98.8 %, Completeness: 95.9 %, Completeness (bb): 95.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.9 % (862 of 899)	95.9 % (446 of 465)	95.2 % (337 of 354)	98.8 % (79 of 80)
Backbone	95.6 % (455 of 476)	95.8 % (158 of 165)	94.9 % (225 of 237)	97.3 % (72 of 74)
Sidechain	94.6 % (469 of 496)	94.3 % (283 of 300)	94.7 % (180 of 190)	100.0 % (6 of 6)
Aromatic	96.3 % (52 of 54)	100.0 % (27 of 27)	92.3 % (24 of 26)	100.0 % (1 of 1)
Methyl	94.8 % (91 of 96)	93.8 % (45 of 48)	95.8 % (46 of 48)

1. SilB(440-521)

GPEHRAVGRI QSIGERSLII AHEAIPSAQW GAMTMEFAAP PAGLPQGLKA GDRVAFSFRL DPHGMATLVT VAPQVQTAGA KP

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	SilB(440-521)	[U-100% 15N]	1 mM
2	MES	natural abundance	50 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0

#	Name	Isotope labeling	Concentration
5	SilB(440-521)	[U-100% 13C; U-100% 15N]	1 mM
6	MES	natural abundance	50 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0

#	Name	Isotope labeling	Concentration
9	SilB(440-521)	[U-100% 15N]	1 mM
10	MES	natural abundance	50 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.77 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.77 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.77 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.77 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.71 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L55, Strand ID: A Detail

Heteronucl. T₁

64 T₁ values in 1 lists
Coherence Sz, Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 273 K, pH 6.0 Detail

Heteronucl. T₂

66 T₂ values in 1 lists
Coherence S(+,-), Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 273 K, pH 6.0 Detail

Heteronucl. NOE

68 NOE values in 1 lists
Value type relative intensities, Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 273 K, pH 6.0 Detail

Heteronucl. T₁/T₂

62 T₁/T₂ values in 1 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 273 K, pH 6.0 Detail

Release date

2011-02-09

Citation

Structural and metal binding characterization of the C-terminal metallochaperone domain of membrane fusion protein SilB from Cupriavidus metallidurans CH34
Bersch, B., Derfoufi, K., De Angelis, F., Auquier, V., Ngonlong Ekende, E., Mergeay, M., Ruysschaert, J., Vandenbussche, G.
Biochemistry (2011), 50, 2194-2204, PubMed 21299248 , DOI 10.1021/bi200005k , Abstract

Related entities 1. SilB(440-521), : 1 entities Detail

Experiments performed 17 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian VNMRS - 600 MHz cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0

#	Name	Isotope labeling	Concentration
5	SilB(440-521)	[U-100% 13C; U-100% 15N]	1 mM
6	MES	natural abundance	50 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

2 2D 1H-13C HSQC

Instrument

Varian VNMRS - 600 MHz cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0

#	Name	Isotope labeling	Concentration
5	SilB(440-521)	[U-100% 13C; U-100% 15N]	1 mM
6	MES	natural abundance	50 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

3 3D HNCO

Instrument

Varian VNMRS - 600 MHz cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0

#	Name	Isotope labeling	Concentration
5	SilB(440-521)	[U-100% 13C; U-100% 15N]	1 mM
6	MES	natural abundance	50 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

4 3D HNCACB

Instrument

Varian VNMRS - 600 MHz cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0

#	Name	Isotope labeling	Concentration
5	SilB(440-521)	[U-100% 13C; U-100% 15N]	1 mM
6	MES	natural abundance	50 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

5 3D HN(CO)CA

Instrument

Varian VNMRS - 600 MHz cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0

#	Name	Isotope labeling	Concentration
5	SilB(440-521)	[U-100% 13C; U-100% 15N]	1 mM
6	MES	natural abundance	50 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

6 3D HN(CO)CACB

Instrument

Varian VNMRS - 600 MHz cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0

#	Name	Isotope labeling	Concentration
5	SilB(440-521)	[U-100% 13C; U-100% 15N]	1 mM
6	MES	natural abundance	50 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

7 3D-intraHNCA

Instrument

Varian VNMRS - 600 MHz cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0

#	Name	Isotope labeling	Concentration
5	SilB(440-521)	[U-100% 13C; U-100% 15N]	1 mM
6	MES	natural abundance	50 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

8 3D-intraHN(CA)CB

Instrument

Varian VNMRS - 600 MHz cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0

#	Name	Isotope labeling	Concentration
5	SilB(440-521)	[U-100% 13C; U-100% 15N]	1 mM
6	MES	natural abundance	50 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

9 2D 1H-15N HADAMAC

Instrument

Varian VNMRS - 600 MHz cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0

#	Name	Isotope labeling	Concentration
5	SilB(440-521)	[U-100% 13C; U-100% 15N]	1 mM
6	MES	natural abundance	50 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

10 3D C(CO)NH

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0

#	Name	Isotope labeling	Concentration
5	SilB(440-521)	[U-100% 13C; U-100% 15N]	1 mM
6	MES	natural abundance	50 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

11 3D 1H-15N NOESY

Instrument

Varian VNMRS - 800 MHz cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	SilB(440-521)	[U-100% 15N]	1 mM
2	MES	natural abundance	50 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

12 3D 1H-13C NOESY

Instrument

Varian VNMRS - 800 MHz cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0

#	Name	Isotope labeling	Concentration
5	SilB(440-521)	[U-100% 13C; U-100% 15N]	1 mM
6	MES	natural abundance	50 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

13 2D 1H-1H NOESY

Instrument

Varian VNMRS - 800 MHz cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0

#	Name	Isotope labeling	Concentration
9	SilB(440-521)	[U-100% 15N]	1 mM
10	MES	natural abundance	50 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

14 T1 relaxation

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	SilB(440-521)	[U-100% 15N]	1 mM
2	MES	natural abundance	50 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

15 T1rho relaxation

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	SilB(440-521)	[U-100% 15N]	1 mM
2	MES	natural abundance	50 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

16 T2 relaxation

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	SilB(440-521)	[U-100% 15N]	1 mM
2	MES	natural abundance	50 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

17 Heteronuclear NOE

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	SilB(440-521)	[U-100% 15N]	1 mM
2	MES	natural abundance	50 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

nullKeywords copper resistance, heavy metal efflux, resistance nodulation cell division, silver resistance, Ag(I)-binding site, apo form, Cu(I)-binding site, CusF ortholog

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.729 seconds)

BMRB: 17266

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. SilB(440-521), : 1 entities Detail

Experiments performed 17 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample