Solution structures of human PIWI-like 1 PAZ domain
CTDVSHKVLR SETVLDFMFN FYHQTEEHKF QEQVSKELIG LVVLTKYNNK TYRVDDIDWD QNPKSTFKKA DGSEVSFLEY YRKQYNQEIT DLKQPVLVSQ PKRRRGPGGT LPGPAMLIPE LCYLTGLTDK MRND
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 83.8 % (1367 of 1631) | 87.6 % (751 of 857) | 75.8 % (479 of 632) | 96.5 % (137 of 142) |
Backbone | 84.9 % (671 of 790) | 99.3 % (266 of 268) | 70.9 % (280 of 395) | 98.4 % (125 of 127) |
Sidechain | 84.9 % (822 of 968) | 82.3 % (485 of 589) | 89.3 % (325 of 364) | 80.0 % (12 of 15) |
Aromatic | 62.1 % (87 of 140) | 64.3 % (45 of 70) | 59.4 % (41 of 69) | 100.0 % (1 of 1) |
Methyl | 100.0 % (132 of 132) | 100.0 % (66 of 66) | 100.0 % (66 of 66) |
1. Human PIWI-like 1 PAZ domain
CTDVSHKVLR SETVLDFMFN FYHQTEEHKF QEQVSKELIG LVVLTKYNNK TYRVDDIDWD QNPKSTFKKA DGSEVSFLEY YRKQYNQEIT DLKQPVLVSQ PKRRRGPGGT LPGPAMLIPE LCYLTGLTDK MRNDSolvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | sodium phosphate | natural abundance | 100 mM | |
2 | sodium chloride | natural abundance | 150 mM | |
3 | DTT | [U-100% 2H] | 5 mM | |
4 | D2O | natural abundance | 100 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | sodium phosphate | natural abundance | 100 mM | |
6 | sodium chloride | natural abundance | 150 mM | |
7 | DTT | [U-100% 2H] | 5 mM | |
8 | D2O | natural abundance | 10 % | |
9 | H2O | natural abundance | 90 % |