NMR structure of the A730 loop of the Neurospora VS ribozyme
Polymer type: polyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 99.0 % (406 of 410) | 98.7 % (222 of 225) | 100.0 % (173 of 173) | 91.7 % (11 of 12) |
Suger, PO4 | 100.0 % (286 of 286) | 100.0 % (156 of 156) | 100.0 % (130 of 130) | |
Nucleobase | 96.8 % (120 of 124) | 95.7 % (66 of 69) | 100.0 % (43 of 43) | 91.7 % (11 of 12) |
Aromatic | 98.2 % (108 of 110) | 98.2 % (54 of 55) | 100.0 % (43 of 43) | 91.7 % (11 of 12) |
1. VS ribozyme SVI RNA (26-MER)
GAGCUGCAGC ACGAAAGUGA CGGCUCSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5, Details 10 mM sodium cacodylate, pH 6.5, 50 mM KCl, 0.05 mM NaN3 and 5 mM MgCl2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | VS ribozyme SVI RNA (26-MER) | natural abundance | 0.4 ~ 1.8 (±0.1) mM | |
2 | H2O | natural abundance | 90 % | |
3 | D2O | natural abundance | 10 % | |
4 | sodium cacodylate | natural abundance | 10 mM | |
5 | KCl | natural abundance | 50 mM | |
6 | NaN3 | natural abundance | 0.05 mM | |
7 | MgCl2 | natural abundance | 5 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5, Details 10 mM sodium cacodylate, pH 6.5, 50 mM KCl, 0.05 mM NaN3 and 5 mM MgCl2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | VS ribozyme SVI RNA (26-MER) | natural abundance | 0.4 ~ 1.8 (±0.1) mM | |
9 | D2O | natural abundance | 100 % | |
10 | sodium cacodylate | natural abundance | 10 mM | |
11 | KCl | natural abundance | 50 mM | |
12 | NaN3 | natural abundance | 0.05 mM | |
13 | MgCl2 | natural abundance | 5 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5, Details 10 mM sodium cacodylate, pH 6.5, 50 mM KCl, 0.05 mM NaN3 and 5 mM MgCl2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
14 | VS ribozyme SVI RNA (26-MER) | [U-15N] | 0.4 ~ 1.8 (±0.1) mM | |
15 | H2O | natural abundance | 90 % | |
16 | D2O | natural abundance | 10 % | |
17 | sodium cacodylate | natural abundance | 10 mM | |
18 | KCl | natural abundance | 50 mM | |
19 | NaN3 | natural abundance | 0.05 mM | |
20 | MgCl2 | natural abundance | 5 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5, Details 10 mM sodium cacodylate, pH 6.5, 50 mM KCl, 0.05 mM NaN3 and 5 mM MgCl2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
21 | VS ribozyme SVI RNA (26-MER) | [U-15N] | 0.4 ~ 1.8 (±0.1) mM | |
22 | D2O | natural abundance | 100 % | |
23 | sodium cacodylate | natural abundance | 10 mM | |
24 | KCl | natural abundance | 50 mM | |
25 | NaN3 | natural abundance | 0.05 mM | |
26 | MgCl2 | natural abundance | 5 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5, Details 10 mM sodium cacodylate, pH 6.5, 50 mM KCl, 0.05 mM NaN3 and 5 mM MgCl2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
27 | VS ribozyme SVI RNA (26-MER) | [U-13C; U-15N] | 0.4 ~ 1.8 (±0.1) mM | |
28 | H2O | natural abundance | 90 % | |
29 | D2O | natural abundance | 10 % | |
30 | sodium cacodylate | natural abundance | 10 mM | |
31 | KCl | natural abundance | 50 mM | |
32 | NaN3 | natural abundance | 0.05 mM | |
33 | MgCl2 | natural abundance | 5 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5, Details 10 mM sodium cacodylate, pH 6.5, 50 mM KCl, 0.05 mM NaN3 and 5 mM MgCl2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
34 | VS ribozyme SVI RNA (26-MER) | [U-13C; U-15N] | 0.4 ~ 1.8 (±0.1) mM | |
35 | D2O | natural abundance | 100 % | |
36 | sodium cacodylate | natural abundance | 10 mM | |
37 | KCl | natural abundance | 50 mM | |
38 | NaN3 | natural abundance | 0.05 mM | |
39 | MgCl2 | natural abundance | 5 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5, Details 10 mM sodium cacodylate, pH 6.5, 50 mM KCl, 0.05 mM NaN3 and 5 mM MgCl2 ~17 mg/mL Pf1 filamentous phage solution (ASLA biotech.)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
40 | VS ribozyme SVI RNA (26-MER) | [U-15N] | 0.4 (±0.1) mM | |
41 | H2O | natural abundance | 90 % | |
42 | D2O | natural abundance | 10 % | |
43 | sodium cacodylate | natural abundance | 10 mM | |
44 | KCl | natural abundance | 50 mM | |
45 | NaN3 | natural abundance | 0.05 mM | |
46 | MgCl2 | natural abundance | 5 mM | |
47 | Pf1 filamentous phage | natural abundance | 17 mg/mL |
Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5, Details 10 mM sodium cacodylate, pH 6.5, 50 mM KCl, 0.05 mM NaN3 and 5 mM MgCl2 ~17 mg/mL Pf1 filamentous phage solution (ASLA biotech.)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
48 | VS ribozyme SVI RNA (26-MER) | [U-13C; U-15N] | 0.4 (±0.1) mM | |
49 | D2O | natural abundance | 100 % | |
50 | sodium cacodylate | natural abundance | 10 mM | |
51 | KCl | natural abundance | 50 mM | |
52 | NaN3 | natural abundance | 0.05 mM | |
53 | MgCl2 | natural abundance | 5 mM | |
54 | Pf1 filamentous phage | natural abundance | 17 mg/mL |