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BMRB: 17293

2L6L

Solution structure of human J-protein co-chaperone Dph4

Authors

Thakur, A., Chitoor, B.S., Atreya, H.S., Silva, P.D.

Assembly

Zn-Dph4

Entity

1. Dph4 (polymer, Thiol state: free and other bound), 155 monomers, 17961.96 Da Detail

MMAVEQMPKK DWYSILGADP SANISDLKQK YQKLILMYHP DKQSTDVPAG TVEECVQKFI EIDQAWKILG NEETKREYDL QRCEDDLRNV GPVDAQVYLE EMSWNEGDHS FYLSCRCGGK YSVSKDEAEE VSLISCDTCS LIIELLHYNH HHHHH

2. ZN (non-polymer, Thiol state: not present), 65.409 Da

Total weight

18027.37 Da

Max. entity weight

17961.96 Da

Entity Connection

na 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS115:SG	2:ZN1:ZN
2	na	sing	1:CYS117:SG	2:ZN1:ZN
3	na	sing	1:CYS136:SG	2:ZN1:ZN
4	na	sing	1:CYS139:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.8 %, Completeness: 80.6 %, Completeness (bb): 87.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	80.6 % (1466 of 1818)	83.3 % (789 of 947)	76.5 % (539 of 705)	83.1 % (138 of 166)
Backbone	87.3 % (803 of 920)	89.4 % (279 of 312)	86.7 % (397 of 458)	84.7 % (127 of 150)
Sidechain	76.2 % (797 of 1046)	80.3 % (510 of 635)	69.9 % (276 of 395)	68.8 % (11 of 16)
Aromatic	3.8 % (6 of 156)	3.8 % (3 of 78)	0.0 % (0 of 75)	100.0 % (3 of 3)
Methyl	98.6 % (144 of 146)	100.0 % (73 of 73)	97.3 % (71 of 73)

1. Dph4

MMAVEQMPKK DWYSILGADP SANISDLKQK YQKLILMYHP DKQSTDVPAG TVEECVQKFI EIDQAWKILG NEETKREYDL QRCEDDLRNV GPVDAQVYLE EMSWNEGDHS FYLSCRCGGK YSVSKDEAEE VSLISCDTCS LIIELLHYNH HHHHH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	Dph4 polypeptide	[U-13C; U-15N]	1 mM
2	Zn	natural abundance	1 mM
3	sodium chloride	natural abundance	80 mM
4	TRIS	natural abundance	20 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
7	Dph4 polypeptide	natural abundance	1 mM
8	Zn	natural abundance	1 mM
9	sodium chloride	natural abundance	80 mM
10	TRIS	natural abundance	20 mM
11	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.49 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.49 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.14 ppm, Outliers: 1 Detail

Release date

2012-01-08

Citation

Structure and mechanistic insights into novel iron-mediated moonlighting functions of human J-protein cochaperone, Dph4
Thakur, A., Chitoor, B., Goswami, A.V., Pareek, G., Atreya, H.S.
J. Biol. Chem. (2012), 287, 13194-13205, PubMed 22367199 , DOI 10.1074/jbc.M112.339655 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: Q6P3W2 released on 2005-05-24
Title DJC24_HUMAN Entity DnaJ homolog subfamily C member 24

Related entities 1. Dph4, : 1 : 2 : 14 entities Detail

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC