Three-dimensional structure of the N-terminal effector PYRIN domain of NLRP12
GHMLRTAGRD GLCRLSTYLE ELEAVELKKF KLYLGTATEL GEGKIPWGSM EKAGPLEMAQ LLITHFGPEE AWRLALSTFE RINRKDLWER GQREDLVRDT VE
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 90.6 % (1096 of 1210) | 92.6 % (586 of 633) | 86.4 % (408 of 472) | 97.1 % (102 of 105) |
Backbone | 95.0 % (576 of 606) | 95.7 % (202 of 211) | 93.9 % (278 of 296) | 97.0 % (96 of 99) |
Sidechain | 87.6 % (610 of 696) | 91.0 % (384 of 422) | 82.1 % (220 of 268) | 100.0 % (6 of 6) |
Aromatic | 56.7 % (51 of 90) | 95.6 % (43 of 45) | 11.9 % (5 of 42) | 100.0 % (3 of 3) |
Methyl | 99.1 % (115 of 116) | 100.0 % (58 of 58) | 98.3 % (57 of 58) |
1. NLRP12 PYD
GHMLRTAGRD GLCRLSTYLE ELEAVELKKF KLYLGTATEL GEGKIPWGSM EKAGPLEMAQ LLITHFGPEE AWRLALSTFE RINRKDLWER GQREDLVRDT VESolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N-labeled NLRP12 PYD sample
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | NLRP12 PYD | [U-99% 15N] | 600 uM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 500 mM | |
4 | TCEP | natural abundance | 0.5 mM | |
5 | H2O | natural abundance | 90 % | |
6 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 13C/15N-labeled NLRP12 PYD sample
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | NLRP12 PYD | [U-99% 13C; U-99% 15N] | 600 uM | |
8 | sodium phosphate | natural abundance | 20 mM | |
9 | sodium chloride | natural abundance | 500 mM | |
10 | TCEP | natural abundance | 0.5 mM | |
11 | H2O | natural abundance | 90 % | |
12 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details Unlabeled NLRP12 PYD sample
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | NLRP12 PYD | natural abundance | 600 uM | |
14 | sodium phosphate | natural abundance | 20 mM | |
15 | sodium chloride | natural abundance | 500 mM | |
16 | TCEP | natural abundance | 0.5 mM | |
17 | D2O | natural abundance | 100 % |