NRC consensus ankyrin repeat protein backbone and sidechain assignments
GHMWGSKDGN TPLHNAAKNG HAEEVKKLLS KGADVNARSK DGNTPLHLAA KNGHAEIVKL LLAKGADVNA RSKDGNTPEH LAKKNGHHEI VKLLDAKGAD VNARSWGSSH HHHHH
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 82.8 % (1051 of 1269) | 80.7 % (532 of 659) | 85.0 % (413 of 486) | 85.5 % (106 of 124) |
Backbone | 89.8 % (614 of 684) | 87.9 % (210 of 239) | 90.1 % (300 of 333) | 92.9 % (104 of 112) |
Sidechain | 77.3 % (532 of 688) | 76.7 % (322 of 420) | 81.3 % (208 of 256) | 16.7 % (2 of 12) |
Aromatic | 13.8 % (11 of 80) | 17.5 % (7 of 40) | 7.9 % (3 of 38) | 50.0 % (1 of 2) |
Methyl | 92.0 % (103 of 112) | 89.3 % (50 of 56) | 94.6 % (53 of 56) |
1. NR1C
GHMWGSKDGN TPLHNAAKNG HAEEVKKLLS KGADVNARSK DGNTPLHLAA KNGHAEIVKL LLAKGADVNA RSKDGNTPEH LAKKNGHHEI VKLLDAKGAD VNARSWGSSH HHHHHSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 25 mM NaPO4- 25 mM NaCl pH 6.5 25C
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | NR1C | [U-100% 13C; U-100% 15N] | 2 mM | |
2 | sodium phosphate | natural abundance | 25 mM | |
3 | sodium chloride | natural abundance | 25 mM | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |