BMRBj - BMREntryMaster

Found 1 Document (3.298 seconds)

BMRB: 17312

2L6J

Tah1 complexed by MEEVD

Authors

Jimenez, B., Ugwu, F., Zhao, R., Orti, L., Houry, W.A., Pineda-Lucena, A.

Assembly

Tah1 complex with C-terminus of Hsp90 (MEEVD)

Entity

1. Tah1 (polymer, Thiol state: all free), 111 monomers, 12511.03 Da Detail

MSQFEKQKEQ GNSLFKQGLY REAVHCYDQL ITAQPQNPVG YSNKAMALIK LGEYTQAIQM CQQGLRYTST AEHVAIRSKL QYRLELAQGA VGSVQIPVVE VDELPEGYDR S

2. MEEVD (polymer, Thiol state: not present), 5 monomers, 621.6569 Da Detail

MEEVD

Total weight

13132.6875 Da

Max. entity weight

12511.03 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.3 %, Completeness: 94.3 %, Completeness (bb): 94.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.3 % (1282 of 1359)	94.9 % (676 of 712)	94.2 % (488 of 518)	91.5 % (118 of 129)
Backbone	94.0 % (647 of 688)	94.9 % (224 of 236)	94.1 % (320 of 340)	92.0 % (103 of 112)
Sidechain	95.0 % (740 of 779)	95.0 % (452 of 476)	95.5 % (273 of 286)	88.2 % (15 of 17)
Aromatic	91.7 % (77 of 84)	97.6 % (41 of 42)	85.7 % (36 of 42)
Methyl	100.0 % (122 of 122)	100.0 % (61 of 61)	100.0 % (61 of 61)

1. Tah1

MSQFEKQKEQ GNSLFKQGLY REAVHCYDQL ITAQPQNPVG YSNKAMALIK LGEYTQAIQM CQQGLRYTST AEHVAIRSKL QYRLELAQGA VGSVQIPVVE VDELPEGYDR S

2. MEEVD

MEEVD

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 8

#	Name	Isotope labeling	Concentration
1	Tah1	[U-99% 15N]	1 mM
2	Ac-MEEVD	natural abundance	2 mM
3	sodium phosphate	natural abundance	25 mM
4	sodium chloride	natural abundance	100 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 8

#	Name	Isotope labeling	Concentration
7	Tah1	[U-99% 13C; U-99% 15N]	1 mM
8	Ac-MEEVD	natural abundance	2 mM
9	sodium phosphate	natural abundance	25 mM
10	sodium chloride	natural abundance	100 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 8

#	Name	Isotope labeling	Concentration
13	Tah1	natural abundance	800 uM
14	Ac-MEEVD	natural abundance	1.6 mM
15	sodium phosphate	natural abundance	25 mM
16	sodium chloride	natural abundance	100 mM
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 8

#	Name	Isotope labeling	Concentration
19	Tah1	[U-98% 13C; U-98% 15N]	1 mM
20	Ac-MEEVD	natural abundance	2 mM
21	sodium phosphate	natural abundance	25 mM
22	sodium chloride	natural abundance	100 mM
23	D2O	natural abundance	100 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2L6J, Strand ID: A, B Detail

Release date

2012-01-03

Citation 1

Structure of minimal tetratricopeptide repeat domain protein Tah1 reveals mechanism of its interaction with Pih1 and Hsp90
Jimenez, B., Ugwu, F., Zhao, R., Orti, L., Makhnevych, T., Pineda-Lucena, A., Houry, W.A.
J. Biol. Chem. (2012), 287, 5698-5709, PubMed 22179618 , DOI 10.1074/jbc.M111.287458 , Abstract

Show more 1 citaion

Citation 2

NMR Assignment of Tah1 protein bound to C-terminus of Hsp90
Jimenez, B., Pantoja-Uceda, D., Hawkins, C., Orti, L., Zhao, R., Houry, W.A., Pineda-Lucena, A.
Biomol. NMR Assignments

Hide non-primary 1 citaion

Related entities 1. Tah1, : 2 : 26 entities Detail

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz It is equipped with a TCI cryo-probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 8

#	Name	Isotope labeling	Concentration
1	Tah1	[U-99% 15N]	1 mM
2	Ac-MEEVD	natural abundance	2 mM
3	sodium phosphate	natural abundance	25 mM
4	sodium chloride	natural abundance	100 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz It is equipped with a TCI cryo-probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 8

#	Name	Isotope labeling	Concentration
1	Tah1	[U-99% 15N]	1 mM
2	Ac-MEEVD	natural abundance	2 mM
3	sodium phosphate	natural abundance	25 mM
4	sodium chloride	natural abundance	100 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

3 2D 1H-1H NOESY

Instrument

Bruker Avance - 900 MHz It is equipped with a TCI cryo-probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 8

#	Name	Isotope labeling	Concentration
13	Tah1	natural abundance	800 uM
14	Ac-MEEVD	natural abundance	1.6 mM
15	sodium phosphate	natural abundance	25 mM
16	sodium chloride	natural abundance	100 mM
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

4 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz It is equipped with a TCI cryo-probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 8

#	Name	Isotope labeling	Concentration
7	Tah1	[U-99% 13C; U-99% 15N]	1 mM
8	Ac-MEEVD	natural abundance	2 mM
9	sodium phosphate	natural abundance	25 mM
10	sodium chloride	natural abundance	100 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

5 3D HNCO

Instrument

Bruker Avance - 600 MHz It is equipped with a TCI cryo-probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 8

#	Name	Isotope labeling	Concentration
7	Tah1	[U-99% 13C; U-99% 15N]	1 mM
8	Ac-MEEVD	natural abundance	2 mM
9	sodium phosphate	natural abundance	25 mM
10	sodium chloride	natural abundance	100 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

6 3D HNCA

Instrument

Bruker Avance - 600 MHz It is equipped with a TCI cryo-probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 8

#	Name	Isotope labeling	Concentration
7	Tah1	[U-99% 13C; U-99% 15N]	1 mM
8	Ac-MEEVD	natural abundance	2 mM
9	sodium phosphate	natural abundance	25 mM
10	sodium chloride	natural abundance	100 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

7 3D HNCACB

Instrument

Bruker Avance - 600 MHz It is equipped with a TCI cryo-probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 8

#	Name	Isotope labeling	Concentration
7	Tah1	[U-99% 13C; U-99% 15N]	1 mM
8	Ac-MEEVD	natural abundance	2 mM
9	sodium phosphate	natural abundance	25 mM
10	sodium chloride	natural abundance	100 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

8 3D HBHA(CO)NH

Instrument

Bruker Avance - 600 MHz It is equipped with a TCI cryo-probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 8

#	Name	Isotope labeling	Concentration
7	Tah1	[U-99% 13C; U-99% 15N]	1 mM
8	Ac-MEEVD	natural abundance	2 mM
9	sodium phosphate	natural abundance	25 mM
10	sodium chloride	natural abundance	100 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

9 3D H(CCO)NH

Instrument

Bruker Avance - 600 MHz It is equipped with a TCI cryo-probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 8

#	Name	Isotope labeling	Concentration
7	Tah1	[U-99% 13C; U-99% 15N]	1 mM
8	Ac-MEEVD	natural abundance	2 mM
9	sodium phosphate	natural abundance	25 mM
10	sodium chloride	natural abundance	100 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

10 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz It is equipped with a TCI cryo-probe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 8

#	Name	Isotope labeling	Concentration
19	Tah1	[U-98% 13C; U-98% 15N]	1 mM
20	Ac-MEEVD	natural abundance	2 mM
21	sodium phosphate	natural abundance	25 mM
22	sodium chloride	natural abundance	100 mM
23	D2O	natural abundance	100 %

11 3D 1H-15N NOESY

Instrument

Bruker Avance - 900 MHz It is equipped with a TCI cryo-probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 8

#	Name	Isotope labeling	Concentration
1	Tah1	[U-99% 15N]	1 mM
2	Ac-MEEVD	natural abundance	2 mM
3	sodium phosphate	natural abundance	25 mM
4	sodium chloride	natural abundance	100 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

12 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 900 MHz It is equipped with a TCI cryo-probe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 8

#	Name	Isotope labeling	Concentration
19	Tah1	[U-98% 13C; U-98% 15N]	1 mM
20	Ac-MEEVD	natural abundance	2 mM
21	sodium phosphate	natural abundance	25 mM
22	sodium chloride	natural abundance	100 mM
23	D2O	natural abundance	100 %

13 2D 15N edited 1H-1H NOESY

Instrument

Bruker Avance - 900 MHz It is equipped with a TCI cryo-probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 8

#	Name	Isotope labeling	Concentration
1	Tah1	[U-99% 15N]	1 mM
2	Ac-MEEVD	natural abundance	2 mM
3	sodium phosphate	natural abundance	25 mM
4	sodium chloride	natural abundance	100 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

14 2D 13C-13C COSY

Instrument

Bruker Avance - 600 MHz It is equipped with a TCI cryo-probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 8

#	Name	Isotope labeling	Concentration
7	Tah1	[U-99% 13C; U-99% 15N]	1 mM
8	Ac-MEEVD	natural abundance	2 mM
9	sodium phosphate	natural abundance	25 mM
10	sodium chloride	natural abundance	100 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

15 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 600 MHz It is equipped with a TCI cryo-probe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 8

#	Name	Isotope labeling	Concentration
19	Tah1	[U-98% 13C; U-98% 15N]	1 mM
20	Ac-MEEVD	natural abundance	2 mM
21	sodium phosphate	natural abundance	25 mM
22	sodium chloride	natural abundance	100 mM
23	D2O	natural abundance	100 %

nullKeywords Protein, tetratricopeptide repeat (TPR)

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Found 1 Document (3.298 seconds)

BMRB: 17312

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Related entities 1. Tah1, : 2 : 26 entities Detail

Experiments performed 15 experiments Detail

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