BMRB: 17333

Deubiquitinating function of ataxin-3: Insights from the solution structure of the Josephin domain

Authors

Mao, Y., Senic-Matuglia, F., Di Fiore, P.P., Polo, S., Hodsdon, M.E., De Camilli, P.

Assembly

JD/UB complex

Entity

1. JD (polymer, Thiol state: not reported), 190 monomers, 21802.43 Da Detail

GPLGSMESIF HEKQEGSLCA QHCLNNLLQG EYFSPVELSS IAHQLDEEER MRMAEGGVTS EDYRTFLQQP SGNMDDSGFF SIQVISNALK VWGLELILFN SPEYQRLRID PINERSFICN YKEHWFTVRK LGKQWFNLNS LLTGPELISD TYLALFLAQL QQEGYSIFVV KGDLPDCEAD QLLQMIRVQQ

2. UB (polymer, Thiol state: not reported), 76 monomers, 8564.731 Da Detail

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

Total weight

30367.16 Da

Max. entity weight

21802.43 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

binding_constants

Binding constant

4 Kd values in 1 lists
Pressure 1 atm, Temperature 298 K, pH 6.4 Detail

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List #1 binding_data, Participants 1. JD, 2. UB,

Sample ID	Exp. name	Binding reaction	Obs. entity ID	Obs. parameter	Value type	Value	Value error
1	2D 1H-15N HSQC	1 + 2 ⇄ 12	1	chemical shifts	Kd	3.4 mM	0.3 mM
2	2D 1H-15N HSQC	1 + 2 ⇄ 12	1	chemical shifts	Kd	3.4 mM	0.3 mM
3	2D 1H-15N HSQC	1 + 2 ⇄ 12	1	chemical shifts	Kd	3.4 mM	0.3 mM
4	2D 1H-15N HSQC	1 + 2 ⇄ 12	1	chemical shifts	Kd	3.4 mM	0.3 mM

Release date

2012-05-21

Citation

Deubiquitinating function of ataxin-3: insights from the solution structure of the Josephin domain
Mao, Y., Senic-Matuglia, F., Di Fiore, P., Polo, S., Hodsdon, M.E., De Camilli, P.
Proc. Natl. Acad. Sci. U. S. A. (2005), 102, 12700-12705, PubMed 16118278 , DOI 10.1073/pnas.0506344102 , Abstract

Related entities 1. JD, : 1 : 5 : 35 entities Detail

Related entities 2. UB, : 92 : 6 : 47 : 182 entities Detail

Interaction partners 1. JD, : 30 interactors Detail

Experiments performed 4 experiments Detail