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BMRB: 17357

3JCU

PsbQ protein

Authors

Hornicakova, M., Muller, N.

Assembly

PsbQ protein

Entity

1. PsbQ protein (polymer, Thiol state: not present), 149 monomers, 16521.55 Da Detail

EARPIVVGPP PPLSGGLPGT ENSDQARDGT LPYTKDRFYL QPLPPTEAAQ RAKVSASEIL NVKQFIDRKA WPSLQNDLRL RASYLRYDLK TVISAKPKDE KKSLQELTSK LFSSIDNLDH AAKIKSPTEA EKYYGQTVSN INEVLAKLG

Formula weight

16521.55 Da

Source organism

Spinacia oleracea

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 81.2 %, Completeness: 52.3 %, Completeness (bb): 75.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	52.3 % (917 of 1753)	43.0 % (397 of 923)	61.0 % (415 of 680)	70.0 % (105 of 150)
Backbone	75.0 % (651 of 868)	68.2 % (199 of 292)	78.9 % (347 of 440)	77.2 % (105 of 136)
Sidechain	37.0 % (380 of 1027)	31.4 % (198 of 631)	47.6 % (182 of 382)	0.0 % (0 of 14)
Aromatic	0.0 % (0 of 94)	0.0 % (0 of 47)	0.0 % (0 of 46)	0.0 % (0 of 1)
Methyl	47.6 % (79 of 166)	44.6 % (37 of 83)	50.6 % (42 of 83)

1. PsbQ protein

EARPIVVGPP PPLSGGLPGT ENSDQARDGT LPYTKDRFYL QPLPPTEAAQ RAKVSASEIL NVKQFIDRKA WPSLQNDLRL RASYLRYDLK TVISAKPKDE KKSLQELTSK LFSSIDNLDH AAKIKSPTEA EKYYGQTVSN INEVLAKLG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.00

#	Name	Isotope labeling	Concentration
1	PsbQ protein	[U-99% 13C; U-99% 15N]	0.5 mM
2	D2O	natural abundance	10 %
3	H2O	natural abundance	90 %
4	sodium phosphate	natural abundance	20 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 1 Detail

Release date

2011-01-31

Citation

Backbone assignment and secondary structure of the PsbQ protein from photosystem II
Horniakova, M., Kohoutova, J., Schlagnitweit, J., Wohlschlager, C., Ettrich, R., Fiala, R., Schoefberger, W., Muller, N.
Biomol. NMR Assign. (2011), 5, 169-175, PubMed 21259076 , DOI 10.1007/s12104-011-9293-6 , Abstract

Related entities 1. PsbQ protein, : 1 : 4 : 26 entities Detail

Interaction partners 1. PsbQ protein, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 750 MHz equipped with TCI cryoprobe, z-gradient, at the FMP institute in Berlin-Buch, Germany