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BMRB: 17401


17401
2L8U
NMR Spectroscopy and Molecular Dynamics Simulation of r(CCGCUGCGG)2 Reveal a Dynamic UU Internal Loop Found in Myotonic Dystrophy Type 1
Authors
Parkesh, R., Fountain, M.A., Disney, M.D.
Assembly
5'-R(*CP*CP*GP*CP*UP*GP*CP*GP*G)-3'
Entity
1. 5'-R(*CP*CP*GP*CP*UP*GP*CP*GP*G)-3' (polymer, Thiol state: not present), 9 monomers, 2925.731 × 2 Da Detail

CCGCUGCGG


Total weight
5851.462 Da
Max. entity weight
2925.731 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 59.3 %, Completeness (bb): 63.0 % Detail
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Polymer type: polyribonucleotide

Total1H
All59.3 % (48 of 81)59.3 % (48 of 81)
Suger, PO463.0 % (34 of 54)63.0 % (34 of 54)
Nucleobase51.9 % (14 of 27)51.9 % (14 of 27)
Aromatic73.7 % (14 of 19)73.7 % (14 of 19)

1. RNA (5'-R(*CP*CP*GP*CP*UP*GP*CP*GP*G)-3')

CCGCUGCGG

Show sample condition
Sample

Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 7.2, Details pH 7.2, 0.1 M NaCl, and 0.05 mM EDTA


#NameIsotope labelingTypeConcentration
1RNA (5'-R(*CP*CP*GP*CP*UP*GP*CP*GP*G)-3')natural abundance1.5 (±0.1) mM
2sodium chloridenatural abundance100 mM

Release date
2011-02-03
Citation
NMR spectroscopy and molecular dynamics simulation of r(CCGCUGCGG)₂ reveal a dynamic UU internal loop found in myotonic dystrophy type 1
Parkesh, R., Fountain, M., Disney, M.D.
Biochemistry (2011), 50, 599-601, PubMed 21204525 , DOI 10.1021/bi101896j ,
Experiments performed 3 experiments Detail
NMR combined restraints 4 contents Detail
Keywords base pair, CUG, hydrogen bonding, RNA, UU mismatch