1H, 15N and 13C resonance assignment of the N-terminal C39-peptidase like domain of the ABC transporter Haemolysin B (HlyB)
GAMANSDSCH KIDYGLYALE ILAQYHNVSV NPEEIKHRFD TDGTGLGLTS WLLAAKSLEL KVKQVKKTID RLNFISLPAL VWREDGRHFI LTKVSKEANR YLIFDLEQRN PRVLEQSEFE ALYQGHIILI ASRSSVAGKL AK
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 99.2 % (1682 of 1695) | 99.2 % (873 of 880) | 99.1 % (657 of 663) | 100.0 % (152 of 152) |
Backbone | 99.1 % (838 of 846) | 99.3 % (287 of 289) | 98.8 % (413 of 418) | 99.3 % (138 of 139) |
Sidechain | 99.0 % (973 of 983) | 98.8 % (584 of 591) | 99.2 % (376 of 379) | 100.0 % (13 of 13) |
Aromatic | 98.5 % (132 of 134) | 98.5 % (66 of 67) | 98.5 % (64 of 65) | 100.0 % (2 of 2) |
Methyl | 98.4 % (179 of 182) | 97.8 % (89 of 91) | 98.9 % (90 of 91) |
1. HlyB C39 domain
GAMANSDSCH KIDYGLYALE ILAQYHNVSV NPEEIKHRFD TDGTGLGLTS WLLAAKSLEL KVKQVKKTID RLNFISLPAL VWREDGRHFI LTKVSKEANR YLIFDLEQRN PRVLEQSEFE ALYQGHIILI ASRSSVAGKL AKSolvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.000
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | C39-137 | [U-13C; U-15N] | 2.0 mM | |
2 | H2O | [U-13C; U-15N] | 93 % | |
3 | D2O | [U-13C; U-15N] | 7 % |
Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.000
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | C39-137 | [U-15N] | 2.5 mM | |
5 | H2O | [U-15N] | 93 % | |
6 | D2O | [U-15N] | 7 % |
Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.000, Details all buffer components are deuterated
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | C39-137 | [U-13C; U-15N] | 2.5 mM | |
8 | H2O | [U-13C; U-15N] | 93 % | |
9 | D2O | [U-13C; U-15N] | 7 % |
Solvent system 100% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.000, Details all buffer components are deuterated
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | C39-137 | [U-13C; U-15N] | 2.5 mM | |
11 | D2O | [U-13C; U-15N] | 100 % |