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BMRB: 17447

2LB6

1H, 15N and 13C resonance assignment of a common Major Urinary Protein of the mouse

Authors

Phelan, M.M., McLean, L., Simpson, D., Hurst, J.L., Beynon, R.J., Lian, L.

Assembly

MUP11

Entity

1. MUP11 (polymer, Thiol state: free and disulfide bound), 176 monomers, 20336.33 Da Detail

MGSSHHHHHH IEGREEASST GRNFNVEKIN GEWHTIILAS DKREKIEDNG NFRLFLEQIH VLENSLVLKF HTVRDEECSE LSMVADKTEK AGEYSVTYDG FNTFTIPKTD YDNFLMAHLI NEKDGETFQL MGLYGREPDL SSDIKERFAQ LCEEHGILRE NIIDLSNANR CLQARE

Formula weight

20336.33 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS78:SG	1:CYS171:SG

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.9 %, Completeness: 85.7 %, Completeness (bb): 92.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.7 % (1763 of 2056)	82.5 % (883 of 1070)	89.3 % (710 of 795)	89.0 % (170 of 191)
Backbone	92.1 % (969 of 1052)	90.3 % (326 of 361)	93.0 % (481 of 517)	93.1 % (162 of 174)
Sidechain	81.1 % (948 of 1169)	78.6 % (557 of 709)	86.5 % (383 of 443)	47.1 % (8 of 17)
Aromatic	61.2 % (109 of 178)	61.8 % (55 of 89)	61.4 % (54 of 88)	0.0 % (0 of 1)
Methyl	98.8 % (168 of 170)	98.8 % (84 of 85)	98.8 % (84 of 85)

1. MUP11

MGSSHHHHHH IEGREEASST GRNFNVEKIN GEWHTIILAS DKREKIEDNG NFRLFLEQIH VLENSLVLKF HTVRDEECSE LSMVADKTEK AGEYSVTYDG FNTFTIPKTD YDNFLMAHLI NEKDGETFQL MGLYGREPDL SSDIKERFAQ LCEEHGILRE NIIDLSNANR CLQARE

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	MUP11	[U-98% 13C; U-98% 15N]	1 mM
2	potassium phosphate	natural abundance	25 mM
3	sodium azide	natural abundance	0.2 %
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.8

#	Name	Isotope labeling	Concentration
6	MUP11	[U-98% 13C; U-98% 15N]	1 mM
7	potassium phosphate	natural abundance	25 mM
8	sodium azide	natural abundance	0.2 %
9	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 1.38 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LB6, Strand ID: A Detail

Release date

2012-08-29

Citation

1H, 15N and 13C resonance assignment of darcin, a mouse major urinary protein
Phelan, M.M., McLean, L., Simpson, D.M., Hurst, J.L., Beynon, R.J., Lian, L.Y.
Biomol. NMR Assign. (2010), 4, 239-241, PubMed 20703836 , DOI 10.1007/s12104-010-9253-6 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 16840 released on 2010-08-18
Title 1H, 15N and 13C resonance assignment of Darcin, a Major Urinary Protein of the mouse

Related entities 1. MUP11, : 1 : 15 : 227 entities Detail

Interaction partners 1. MUP11, : 5 interactors Detail

More details for 5 interactors identified by 1 citations