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Found 1 Document (3.427 seconds)

BMRB: 17466

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Solution structure of the RING finger-like domain of Retinoblastoma Binding Protein-6 (RBBP6)

Authors

Kappo, M.A., Ab, E., Atkinson, R.A., Faro, A., Muleya, V., Mulaudzi, T., Poole, J.O., Mckenzie, J.M., PUGH, D.JOHN.RHYDWYN.

Assembly

Solution structure of the RING finger-like domain of Retinoblastoma Binding Protein-6 (RBBP6)

Entity

1. E3 UBIQUITIN-PROTEIN LIGASE RBBP6 (polymer, Thiol state: all disulfide bound), 92 monomers, 10229.51 × 2 Da Detail

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2. ZN (non-polymer), 65.409 × 2 Da

Total weight

20589.838 Da

Max. entity weight

10229.51 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 89.4 %, Completeness (bb): 82.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.4 % (922 of 1031)	97.6 % (524 of 537)	76.9 % (307 of 399)	95.8 % (91 of 95)
Backbone	82.8 % (447 of 540)	96.7 % (176 of 182)	69.5 % (189 of 272)	95.3 % (82 of 86)
Sidechain	97.1 % (562 of 579)	98.0 % (348 of 355)	95.3 % (205 of 215)	100.0 % (9 of 9)
Aromatic	81.8 % (36 of 44)	100.0 % (22 of 22)	63.6 % (14 of 22)
Methyl	100.0 % (98 of 98)	100.0 % (49 of 49)	100.0 % (49 of 49)

1. E3 UBIQUITIN-PROTEIN LIGASE RBBP6

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Show sample condition

Sample

Solvent system 95% H2O, 5% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.000, Details 0.7 mM

#	Name	Isotope labeling	Type	Concentration
1	E3 UBIQUITIN-PROTEIN LIGASE RBBP6			0.7 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
¹H	HDO	proton	4.812 ppm	internal	indirect	1.0
¹⁵N	Liquid NH3	protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
¹H	HDO	proton	4.812 ppm	internal	indirect	1.0
¹⁵N	Liquid NH3	protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
¹H	HDO	proton	4.812 ppm	internal	indirect	1.0
¹⁵N	Liquid NH3	protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 1 Detail

Release date

2011-12-11

Citation

Solution structure of RING finger-like domain of retinoblastoma-binding protein-6 (RBBP6) suggests it functions as a U-box
Kappo, M.A., Ab, E., Hassem, F., Atkinson, R., Faro, A., Muleya, V., Mulaudzi, T., Poole, J.O., McKenzie, J.M., Chibi, M., Moolman-Smook, J.C., Rees, D., Pugh, D.J.R.
J. Biol. Chem. (2012), 287, 7146-7158, PubMed 22130672 , DOI 10.1074/jbc.M110.217059 , Abstract

Related entities 1. E3 UBIQUITIN-PROTEIN LIGASE RBBP6, : 1 : 5 : 50 entities Detail

Interaction partners 1. E3 UBIQUITIN-PROTEIN LIGASE RBBP6, : 7 interactors Detail

More details for 7 interactors identified by 1 citations

Experiments performed 5 experiments Detail

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr17466_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr17466_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list	Warning	1027		100.0 (chain: 1, length: 92)
1	Chemical shift references	chemical_shift_reference_1	Warning	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 92, Sequence coverage: 100.0%
- Total number of assignments
  - ¹³C chemical shifts: 300
  - ¹H chemical shifts: 631
  - ¹⁵N chemical shifts: 96
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
36	TYR	HH	5.979
42	ARG	HH11	7.006
42	ARG	HH21	6.665
92	GLN	CD	21.343

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	399	299	74.9
¹H chemical shifts	537	528	98.3
¹⁵N chemical shifts	99	96	97.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	184	92	50.0
¹H chemical shifts	182	179	98.4
¹⁵N chemical shifts	86	83	96.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	215	207	96.3
¹H chemical shifts	355	349	98.3
¹⁵N chemical shifts	13	13	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	50	50	100.0
¹H chemical shifts	50	50	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	22	14	63.6
¹H chemical shifts	22	22	100.0

Keywords E3 ligase, LIGASE, mRNA processing, mRNA splicing, PACT, RBBP6, Retinoblastoma Binding Protein-6, RING finger, U-Box

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Found 1 Document (3.427 seconds)

BMRB: 17466

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. E3 UBIQUITIN-PROTEIN LIGASE RBBP6, : 1 : 5 : 50 entities Detail

Interaction partners 1. E3 UBIQUITIN-PROTEIN LIGASE RBBP6, : 7 interactors Detail

Experiments performed 5 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 4 contents Detail