1H, 13C, and 15N Chemical Shift Assignments for the FF domain L24A mutant
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 36.5 % (319 of 874) | 27.3 % (127 of 466) | 37.9 % (125 of 330) | 85.9 % (67 of 78) |
Backbone | 76.6 % (317 of 414) | 91.2 % (125 of 137) | 59.5 % (125 of 210) | 100.0 % (67 of 67) |
Sidechain | 0.9 % (5 of 530) | 0.6 % (2 of 329) | 1.6 % (3 of 190) | 0.0 % (0 of 11) |
Aromatic | 0.0 % (0 of 68) | 0.0 % (0 of 34) | 0.0 % (0 of 32) | 0.0 % (0 of 2) |
Methyl | 1.8 % (1 of 56) | 0.0 % (0 of 28) | 3.6 % (1 of 28) |
1. FF Domain L24A
SQPAKKTYTW NTKEEAKQAF KEALKEKRVP SNASWEQAMK MIINDPRYSA LAKLSEKKQA FNAYKVQTEKSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | FF_Domain_L24A | [U-13C; U-15N; U-2H] | 1.0 ~ 2.0 mM | |
2 | sodium chloride | natural abundance | 100 mM | |
3 | sodium acetate | natural abundance | 50 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | FF_Domain_L24A | [U-100% 13C; U-100% 15N; U-50% 2H] | 1.0 ~ 2.0 mM | |
5 | sodium chloride | natural abundance | 100 mM | |
6 | sodium acetate | natural abundance | 50 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | FF_Domain_L24A | [U-13C; U-15N; U-2H] | 1.0 ~ 2.0 mM | |
8 | sodium chloride | natural abundance | 100 mM | |
9 | sodium acetate | natural abundance | 50 mM | |
10 | C12E5/n-hexanol | natural abundance | 5 % |