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BMRB: 17503

2MZW

Staphylococcus aureus FusB

Authors

Cox, G., O'Neill, A.J., Thompson, G.S., Kalverda, A.P., Homans, S.W., Tomlinson, J.H., Edwards, T.A., Jenkins, H.

Assembly

fusb

Entity

1. fusb (polymer, Thiol state: all free), 233 monomers, 27309.00 Da Detail

MGSSHHHHHH SSGLVPNGSH MKTMIYPHQY NYIRSVILRL KNVYKTVNDK ETVKVIQSET YNDINEIFGH IDDDIEESLK VLMNIRLSNK EIEAILNKFL EYVVPFELPS PQKLQKVFKK VKKIKIPQFE EYDLKVSSFV GWNELASNRK YIIYYDEKKQ LKGLYGEISN QVVKGFCTIC NKESNVSLFM KKSKTNSDGQ YVKKGDYICR DSIHCNKQLT DINQFYNFID KLD

Formula weight

27309.0 Da

Source organism

Staphylococcus aureus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 79.0 %, Completeness: 34.9 %, Completeness (bb): 64.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	34.9 % (1012 of 2896)	18.7 % (285 of 1521)	49.3 % (552 of 1119)	68.4 % (175 of 256)
Backbone	64.1 % (889 of 1386)	42.1 % (198 of 470)	75.2 % (518 of 689)	76.2 % (173 of 227)
Sidechain	16.7 % (289 of 1733)	8.4 % (88 of 1051)	30.5 % (199 of 653)	6.9 % (2 of 29)
Aromatic	3.0 % (8 of 264)	3.0 % (4 of 132)	3.1 % (4 of 131)	0.0 % (0 of 1)
Methyl	11.8 % (28 of 238)	11.8 % (14 of 119)	11.8 % (14 of 119)

1. fusb

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.0 K, pH 8.000

#	Name	Isotope labeling	Concentration
1	fusb	[[U-95% 13C; U-95% 15N; U-95% 2H]]	0.3 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	Tris-HCl	natural abundance	20 mM
5	NaCl	natural abundance	300 mM
6	DTT	natural abundance	1 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.0 K, pH 8.000

#	Name	Isotope labeling	Concentration
7	fusb	[U-15N]-Val	0.78 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %
10	Tris-HCl	natural abundance	20 mM
11	NaCl	natural abundance	300 mM
12	DTT	natural abundance	1 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.0 K, pH 8.000

#	Name	Isotope labeling	Concentration
13	fusb	[U-15N]-Ile	0.72 mM
14	H2O	natural abundance	90 %
15	D2O	natural abundance	10 %
16	Tris-HCl	natural abundance	20 mM
17	NaCl	natural abundance	300 mM
18	DTT	natural abundance	1 mM

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.0 K, pH 8.000

#	Name	Isotope labeling	Concentration
19	fusb	[U-15N]-Tyr	0.68 mM
20	H2O	natural abundance	90 %
21	D2O	natural abundance	10 %
22	Tris-HCl	natural abundance	20 mM
23	NaCl	natural abundance	300 mM
24	DTT	natural abundance	1 mM

Sample #5

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.0 K, pH 8.000

#	Name	Isotope labeling	Concentration
25	fusb	[U-15N]-Leu	0.8 mM
26	H2O	natural abundance	90 %
27	D2O	natural abundance	10 %
28	Tris-HCl	natural abundance	20 mM
29	NaCl	natural abundance	300 mM
30	DTT	natural abundance	1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.85 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.85 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.26 ppm, Outliers: 3 Detail

Release date

2012-02-16

Citation

Ribosome clearance by FusB-type proteins mediates resistance to the antibiotic fusidic acid
Cox, G., Thompson, G.S., Jenkins, H.T., Peske, F., Savelsbergh, A., Rodnina, M.V., Wintermeyer, W., Homans, S.W., Edwards, T.A., O'Neill, A.J.
Proc. Natl. Acad. Sci. U. S. A. (2012), 109, 2102-2107, PubMed 22308410 , DOI 10.1073/pnas.1117275109 , Abstract

Related entities 1. fusb, : 1 : 1 : 4 entities Detail

Experiments performed 11 experiments Detail

1 3D HNCA