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BMRB: 17504

2LA5

RNA Duplex-Quadruplex Junction Complex with FMRP RGG peptide

Authors

Phan, A., Kuryavyi, V., Darnell, J., Serganov, A., Majumdar, A., Ilin, S., Darnell, R., Patel, D.

Assembly

RNA Duplex-Quadruplex Junction Complex with FMRP RGG peptide

Entity

1. RNA (36-MER) (polymer), 36 monomers, 11868.00 Da Detail

GCUGCGGUGU GGAAGGAGUG GCUGGGUUGC GCAGCG

2. RNA Duplex-Quadruplex Junction Complex with FMRP RGG peptide, entity 2 (polymer), 17 monomers, 1751.875 Da Detail

GPRRGDGRRR GGGGRGQ

Total weight

13619.875 Da

Max. entity weight

11868.0 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.8 %, Completeness (bb): 98.8 % Detail

Polymer type: polyribonucleotide polypeptide(L)

	Total	¹H
All	96.8 % (391 of 404)	96.8 % (391 of 404)
Backbone	98.8 % (254 of 257)	98.8 % (254 of 257)
Sidechain	93.2 % (137 of 147)	93.2 % (137 of 147)
Aromatic	92.4 % (73 of 79)	92.4 % (73 of 79)

1. RNA (36-MER)

GCUGCGGUGU GGAAGGAGUG GCUGGGUUGC GCAGCG

2. entity 2

GPRRGDGRRR GGGGRGQ

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O and 100%D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	RNA (36-MER)	natural abundance		1 mM
2	Peptide	natural abundance		1 mM

Sample #2

Solvent system 90% H2O/10% D2O and 100%D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
3	RNA (36-MER)	[U-100% 13C; U-100% 15N]		0.5 mM
4	Peptide	natural abundance		0.5 mM

Sample #3

Solvent system 90% H2O/10% D2O and 100%D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
5	RNA (36-MER)	natural abundance		0.5 mM
6	Peptide	[U-100% 13C; U-100% 15N]		0.5 mM

Sample #4

Solvent system 90% H2O/10% D2O and 100%D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
7	RNA (36-MER)	[U-100% 13C; U-100% 15N]		0.2-0.5 mM
8	Peptide	[U-100% 13C; U-100% 15N]		0.2-0.5 mM

Sample #5

Solvent system 90% H2O/10% D2O and 100%D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
9	RNA (36-MER)	[U-13C; U-15N]-Gua		0.5 mM
10	Peptide	natural abundance		0.5 mM

Sample #6

Solvent system 90% H2O/10% D2O and 100%D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
11	RNA (36-MER)	[U-13C; U-15N]-Ade		0.2 mM
12	Peptide	natural abundance		0.2 mM

Sample #7

Solvent system 90% H2O/10% D2O and 100%D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
13	RNA (36-MER)	[U-13C; U-15N]-Cyt		0.2 mM
14	Peptide	natural abundance		0.2 mM

Sample #8

Solvent system 90% H2O/10% D2O and 100%D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
15	RNA (36-MER)	[U-13C; U-15N]-Ura		0.2 mM
16	Peptide	natural abundance		0.2 mM

Sample #9

Solvent system 90% H2O/10% D2O and 100%D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
17	RNA (36-MER)	rG-to-dG and rU-to-dU 2% 15N-labelled		0.2 mM
18	Peptide	natural abundance		0.2 mM

Protein Blocks Logo

Calculated from 10 models in PDB: 2LA5, Strand ID: B Detail

Release date

2011-06-06

Citation

Structure-function studies of FMRP RGG peptide recognition of an RNA duplex-quadruplex junction
Phan, A., Kuryavyi, V., Darnell, J.C., Serganov, A., Majumdar, A., Ilin, S., Raslin, T., Polonskaia, A., Chen, C., Clain, D., Darnell, R.B., Patel, D.J.
Nat. Struct. Mol. Biol. (2011), 18, 796-804, PubMed 21642970 , DOI 10.1038/nsmb.2064 , Abstract

Related entities 1. RNA (36-MER), : 1 : 3 entities Detail

Related entities 2. RNA Duplex-Quadruplex Junction Complex with FMRP RGG peptide, entity 2, : 1 entities Detail

Experiments performed 45 experiments Detail

1 JR