BMRBj - BMREntryMaster

Found 1 Document (0.791 seconds)

BMRB: 17512

2LZN

1H, 13C, and 15N resonance assignments for S. aureus primase C-terminal domain

Authors

Shortridge, M.D., Griep, M.A., Powers, R.

Assembly

primase CTD

Entity

1. primase CTD (polymer, Thiol state: not present), 143 monomers, 17290.79 Da Detail

FDNLSRQEKA ERAFLKHLMR DKDTFLNYYE SVDKDNFTNQ HFKYVFEVLH DFYAENDQYN ISDAVQYVNS NELRETLISL EQYNLNDEPY ENEIDDYVNV INEKGQETIE SLNHKLREAT RIGDVELQKY YLQQIVAKNK ERM

Formula weight

17290.79 Da

Source organism

Staphylococcus aureus

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 80.1 %, Completeness (bb): 92.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	80.1 % (1413 of 1763)	77.5 % (716 of 924)	83.1 % (559 of 673)	83.1 % (138 of 166)
Backbone	92.9 % (795 of 856)	91.3 % (262 of 287)	93.2 % (398 of 427)	95.1 % (135 of 142)
Sidechain	71.8 % (752 of 1048)	71.3 % (454 of 637)	76.2 % (295 of 387)	12.5 % (3 of 24)
Aromatic	31.6 % (55 of 174)	33.3 % (29 of 87)	29.9 % (26 of 87)
Methyl	90.6 % (125 of 138)	88.4 % (61 of 69)	92.8 % (64 of 69)

1. primase CTD

FDNLSRQEKA ERAFLKHLMR DKDTFLNYYE SVDKDNFTNQ HFKYVFEVLH DFYAENDQYN ISDAVQYVNS NELRETLISL EQYNLNDEPY ENEIDDYVNV INEKGQETIE SLNHKLREAT RIGDVELQKY YLQQIVAKNK ERM

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
1	primase CTD	[U-100% 13C; U-100% 15N]		1.4 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.38 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.38 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.13 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.42 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LZN, Strand ID: A Detail

Release date

2011-06-06

Citation

¹H, ¹³C, and ¹⁵N NMR assignments for the helicase interaction domain of Staphylococcus aureus DnaG primase
Shortridge, M.D., Griep, M.A., Powers, R.
Biomol. NMR Assign. (2012), 6, 35-38, PubMed 21644056 , DOI 10.1007/s12104-011-9320-7 , Abstract

Related entities 1. primase CTD, : 1 : 5 : 5 entities Detail

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC