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Pseudouridine_ASL_Tyr
Authors
Denmon, A.P., Wang, J., Nikonowicz, E.P.
Assembly
Anticodon Stem-loop of Bacillus subtilis tRNATYR
Entity
1. Anticodon Stem-loop of Bacillus subtilis tRNATYR (polymer, Thiol state: not present), 17 monomers, 5505.273 Da Detail

GGGGACUGUA AAXCCCC


Formula weight
5505.273 Da
Source organism
Bacillus subtilis
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 94.1 %, Completeness: 41.8 %, Completeness (bb): 33.9 % Detail

Polymer type: polyribonucleotide

Total1H13C31P
All41.8 % (110 of 263)43.6 % (61 of 140)31.8 % (34 of 107)93.8 % (15 of 16)
Suger, PO433.9 % (65 of 192)36.5 % (35 of 96)18.8 % (15 of 80)93.8 % (15 of 16)
Nucleobase63.4 % (45 of 71)59.1 % (26 of 44)70.4 % (19 of 27)
Aromatic73.8 % (45 of 61)76.5 % (26 of 34)70.4 % (19 of 27)

1. tRNATYR

GGGGACUGUA AAXCCCC

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.3


#NameIsotope labelingTypeConcentration
1Pseudouridine_ASL_Tyrnatural abundance2.0 mM
2potassium chloridenatural abundance10 mM
3potassium phosphatenatural abundance10 mM
4EDTAnatural abundance0.02 mM
5H2Onatural abundance90 %
6D2Onatural abundance10 %

Release date
2011-08-01
Citation
Conformation effects of base modification on the anticodon stem-loop of Bacillus subtilis tRNA(Tyr)
Denmon, A.P., Wang, J., Nikonowicz, E.P.
J. Mol. Biol. (2011), 412, 285-303, PubMed 21782828 , DOI 10.1016/j.jmb.2011.07.010 ,
Related entities 1. Anticodon Stem-loop of Bacillus subtilis tRNATYR, : 1 : 3 entities Detail
Experiments performed 3 experiments Detail
NMR combined restraints 6 contents Detail
Keywords Pseudouridine, Tyrosyl-tRNA