Third WW domain of human Nedd4L in complex with doubly phosphorylated human smad3 derived peptide.
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 56.5 % (355 of 628) | 80.1 % (266 of 332) | 27.2 % (67 of 246) | 44.0 % (22 of 50) |
Backbone | 57.8 % (170 of 294) | 85.9 % (85 of 99) | 41.4 % (63 of 152) | 51.2 % (22 of 43) |
Sidechain | 56.0 % (214 of 382) | 77.7 % (181 of 233) | 23.2 % (33 of 142) | 0.0 % (0 of 7) |
Aromatic | 50.0 % (40 of 80) | 100.0 % (40 of 40) | 0.0 % (0 of 38) | 0.0 % (0 of 2) |
Methyl | 2.9 % (1 of 34) | 0.0 % (0 of 17) | 5.9 % (1 of 17) |
1. NEDD4LWW3
GAMEQSFLPP GWEMRIAPNG RPFFYDHNTK TTTWEDPRLK FPVH2. SMAD3
AGXPNLXPNPSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 285 K, pH 7, Details Unlabeled sample
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | NEDD4LWW3 | natural abundance | 1 mM | |
2 | SMAD3 | natural abundance | 3 mM | |
3 | sodium phosphate | natural abundance | 20 mM | |
4 | sodium chloride | natural abundance | 100 mM | |
5 | sodium azide | natural abundance | 2 mM | |
6 | H2O | natural abundance | 90 % | |
7 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 285 K, pH 7, Details 15N labeled sample
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | NEDD4LWW3 | [U-100% 15N] | 1 mM | |
9 | SMAD3 | natural abundance | 3 mM | |
10 | sodium phosphate | natural abundance | 20 mM | |
11 | sodium chloride | natural abundance | 100 mM | |
12 | sodium azide | natural abundance | 2 mM | |
13 | H2O | natural abundance | 90 % | |
14 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 285 K, pH 7, Details 15N,13C labeled sample
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
15 | NEDD4LWW3 | [U-100% 13C; U-100% 15N] | 1 mM | |
16 | SMAD3 | natural abundance | 3 mM | |
17 | sodium phosphate | natural abundance | 20 mM | |
18 | sodium chloride | natural abundance | 100 mM | |
19 | sodium azide | natural abundance | 2 mM | |
20 | H2O | natural abundance | 90 % | |
21 | D2O | natural abundance | 10 % |