DNA / RNA Hybrid containing a central stereo specific Rp borano phosphate linkage
Polymer type: polydeoxyribonucleotide polyribonucleotide
Total | 1H | 13C | 31P | |
---|---|---|---|---|
All | 62.3 % (147 of 236) | 72.2 % (114 of 158) | 29.5 % (18 of 61) | 88.2 % (15 of 17) |
Suger, PO4 | 58.1 % (100 of 172) | 69.1 % (76 of 110) | 20.0 % (9 of 45) | 88.2 % (15 of 17) |
Nucleobase | 73.4 % (47 of 64) | 79.2 % (38 of 48) | 56.3 % (9 of 16) | |
Aromatic | 86.3 % (44 of 51) | 100.0 % (35 of 35) | 56.3 % (9 of 16) | |
Methyl | 100.0 % (3 of 3) | 100.0 % (3 of 3) |
1. DNA (5'-D(*AP*TP*GP*GP*TP*BGR*CP*TP*C)-3')
ATGGTXCTC2. RNA (5'-R(*GP*AP*GP*CP*AP*CP*CP*AP*U)-3')
GAGCACCAUSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DNA_chain_A | natural abundance | 1.0 mM | |
2 | RNA_chain_B | natural abundance | 1.0 mM | |
3 | sodium chloride | natural abundance | 50 mM | |
4 | sodium phosphate | natural abundance | 10 mM | |
5 | EDTA | natural abundance | 0.5 mM | |
6 | DSS | natural abundance | 0.3 uM | |
7 | D2O | natural abundance | 10 % | |
8 | H2O | natural abundance | 90 % |
Solvent system 99.999 % D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | DNA_chain_A | natural abundance | 1.0 mM | |
10 | RNA_chain_B | natural abundance | 1.0 mM | |
11 | sodium chloride | natural abundance | 50 mM | |
12 | sodium phosphate | natural abundance | 10 mM | |
13 | EDTA | natural abundance | 0.5 mM | |
14 | DSS | natural abundance | 0.3 uM | |
15 | D2O | natural abundance | 99.999 % |