Structure of the first domain of human Smurf1 in complex with a doubly phosphorylated human Smad1 derived peptide.
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 52.4 % (264 of 504) | 73.6 % (192 of 261) | 26.4 % (52 of 197) | 43.5 % (20 of 46) |
Backbone | 53.5 % (137 of 256) | 75.3 % (67 of 89) | 39.4 % (50 of 127) | 50.0 % (20 of 40) |
Sidechain | 52.3 % (150 of 287) | 72.7 % (125 of 172) | 22.9 % (25 of 109) | 0.0 % (0 of 6) |
Aromatic | 28.6 % (16 of 56) | 57.1 % (16 of 28) | 0.0 % (0 of 27) | 0.0 % (0 of 1) |
Methyl | 44.7 % (17 of 38) | 89.5 % (17 of 19) | 0.0 % (0 of 19) |
1. human Smurf1
GAMELPEGYE QRTTVQGQVY FLHTQTGVST WHDPRI2. doubly phosphorylated human Smad1 derived peptide
TSXDPGXPFQSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 285 K, pH 7, Details Unlabeled sample
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Smurf1 | natural abundance | 1 mM | |
2 | Smad1 | natural abundance | 3 mM | |
3 | sodium phosphate | natural abundance | 20 mM | |
4 | sodium chloride | natural abundance | 100 mM | |
5 | sodium azide | natural abundance | 2 mM | |
6 | H2O | natural abundance | 90 % | |
7 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 285 K, pH 7, Details 15N labeled sample
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | Smurf1 | [U-100% 15N] | 1 mM | |
9 | Smad1 | natural abundance | 3 mM | |
10 | sodium phosphate | natural abundance | 20 mM | |
11 | sodium chloride | natural abundance | 100 mM | |
12 | sodium azide | natural abundance | 2 mM | |
13 | H2O | natural abundance | 90 % | |
14 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 285 K, pH 7, Details 15N,13C labeled sample
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
15 | Smurf1 | [U-100% 13C; U-100% 15N] | 1 mM | |
16 | Smad1 | natural abundance | 3 mM | |
17 | sodium phosphate | natural abundance | 20 mM | |
18 | sodium chloride | natural abundance | 100 mM | |
19 | sodium azide | natural abundance | 2 mM | |
20 | H2O | natural abundance | 90 % | |
21 | D2O | natural abundance | 10 % |