Structure of the second domain of human Nedd4L in complex with a doubly phosphorylated human Smad3 derived peptide.
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 74.6 % (402 of 539) | 93.0 % (265 of 285) | 49.8 % (103 of 207) | 72.3 % (34 of 47) |
Backbone | 71.4 % (190 of 266) | 93.5 % (86 of 92) | 52.6 % (70 of 133) | 82.9 % (34 of 41) |
Sidechain | 77.7 % (244 of 314) | 92.7 % (179 of 193) | 56.5 % (65 of 115) | 0.0 % (0 of 6) |
Aromatic | 40.4 % (21 of 52) | 80.8 % (21 of 26) | 0.0 % (0 of 24) | 0.0 % (0 of 2) |
Methyl | 84.4 % (27 of 32) | 100.0 % (16 of 16) | 68.8 % (11 of 16) |
1. second domain of human Nedd4L
GLPSGWEERK DAKGRTYYVN HNNRTTTWTR PIMQL2. doubly phosphorylated human Smad3
EXPPPGYLSE DGSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 285 K, pH 7, Details Unlabeled sample
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Nedd4L | natural abundance | 1 mM | |
2 | SMAD3 | natural abundance | 3 mM | |
3 | sodium phosphate | natural abundance | 20 mM | |
4 | sodium chloride | natural abundance | 100 mM | |
5 | sodium azide | natural abundance | 2 mM | |
6 | H2O | natural abundance | 90 % | |
7 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 285 K, pH 7, Details 15N labeled sample
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | Nedd4L | [U-100% 15N] | 1 mM | |
9 | SMAD3 | natural abundance | 3 mM | |
10 | sodium phosphate | natural abundance | 20 mM | |
11 | sodium chloride | natural abundance | 100 mM | |
12 | sodium azide | natural abundance | 2 mM | |
13 | H2O | natural abundance | 90 % | |
14 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 285 K, pH 7, Details 15N,13C labeled sample
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
15 | Nedd4L | [U-100% 13C; U-100% 15N] | 1 mM | |
16 | SMAD3 | natural abundance | 3 mM | |
17 | sodium phosphate | natural abundance | 20 mM | |
18 | sodium chloride | natural abundance | 100 mM | |
19 | sodium azide | natural abundance | 2 mM | |
20 | H2O | natural abundance | 90 % | |
21 | D2O | natural abundance | 10 % |