Structure of a yeast RNase III dsRBD complex with a non-canonical RNA substrate provides new insights into substrate recognition
Polymer type: polypeptide(L) DNA/RNA hybrid
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 55.7 % (854 of 1533) | 54.4 % (441 of 810) | 53.3 % (326 of 612) | 78.4 % (87 of 111) |
Backbone | 57.1 % (506 of 886) | 46.7 % (176 of 377) | 58.8 % (248 of 422) | 94.3 % (82 of 87) |
Sidechain | 58.8 % (429 of 729) | 61.2 % (265 of 433) | 58.5 % (159 of 272) | 20.8 % (5 of 24) |
Aromatic | 8.9 % (17 of 192) | 17.7 % (17 of 96) | 0.0 % (0 of 78) | 0.0 % (0 of 18) |
Methyl | 92.9 % (104 of 112) | 94.6 % (53 of 56) | 91.1 % (51 of 56) |
1. yeast RNase III dsRBD
GSLDMNAKRQ LYSLIGYASL RLHYVTVKKP TAVDPNSIVE CRVGDGTVLG TGVGRNIKIA GIRAAENALR DKKMLDFYAK QRAAIPRSES2. non-canonical RNA substrate
GGGAUACCAU GUUCAAGUGA ACGUGGUAUC UCSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity_1 | [U-100% 13C; U-100% 15N] | 0.8 ~ 1.0 mM | |
2 | entity_2 | natural abundance | 1.0 ~ 2.0 mM | |
3 | NaCl | natural abundance | 150 mM | |
4 | phosphate buffer | natural abundance | 20 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | entity_1 | [U-100% 13C; U-100% 15N] | 0.8 ~ 1.0 mM | |
6 | entity_2 | natural abundance | 1.0 ~ 2.0 mM | |
7 | NaCl | natural abundance | 150 mM | |
8 | phosphate buffer | natural abundance | 20 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | entity_1 | natural abundance | 0.8 ~ 1.0 mM | |
10 | entity_2 | [U-100% 13C; U-100% 15N] | 1.0 ~ 2.0 mM | |
11 | NaCl | natural abundance | 150 mM | |
12 | phosphate buffer | natural abundance | 20 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | entity_1 | natural abundance | 0.8 ~ 1.0 mM | |
14 | entity_2 | [U-100% 13C; U-100% 15N] | 1.0 ~ 2.0 mM | |
15 | NaCl | natural abundance | 150 mM | |
16 | phosphate buffer | natural abundance | 20 mM |