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Solution structure of the S. cerevisiae H/ACA RNP protein Nhp2p-S82W mutant
Authors
Koo, B., Park, C., Fernandez, C.F., Chim, N., Ding, Y., Chanfreau, G., Feigon, J.
Assembly
H/ACA RNP protein Nhp2p-S82W mutant
Entity
1. H/ACA RNP protein Nhp2p-S82W mutant (polymer, Thiol state: all free), 121 monomers, 13278.42 Da Detail

SKKLNKKVLK TVKKASKAKN VKRGVKEVVK ALRKGEKGLV VIAGDIWPAD VISHIPVLCE DHSVPYIFIP SKQDLGAAGA TKRPTSVVFI VPGSNKKKDG KNKEEEYKES FNEVVKEVQA L


Formula weight
13278.42 Da
Source organism
Saccharomyces cerevisiae
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 97.5 %, Completeness: 70.3 %, Completeness (bb): 85.6 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All70.3 % (1022 of 1453)68.4 % (524 of 766)67.9 % (383 of 564)93.5 % (115 of 123)
Backbone85.6 % (611 of 714)91.4 % (223 of 244)78.3 % (278 of 355)95.7 % (110 of 115)
Sidechain60.7 % (517 of 852)57.7 % (301 of 522)65.5 % (211 of 322)62.5 % (5 of 8)
Aromatic18.2 % (12 of 66)33.3 % (11 of 33) 0.0 % (0 of 32)100.0 % (1 of 1)
Methyl83.1 % (123 of 148)93.2 % (69 of 74)73.0 % (54 of 74)

1. Nhp2p-S82W

SKKLNKKVLK TVKKASKAKN VKRGVKEVVK ALRKGEKGLV VIAGDIWPAD VISHIPVLCE DHSVPYIFIP SKQDLGAAGA TKRPTSVVFI VPGSNKKKDG KNKEEEYKES FNEVVKEVQA L

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4


#NameIsotope labelingTypeConcentration
1HEPES[U-13C; U-15N]20 mM
2potassium chloridenatural abundance200 mM
3DTTnatural abundance1 mM
4H2Onatural abundance90 %
5D2Onatural abundance10 %
Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4


#NameIsotope labelingTypeConcentration
6HEPES[U-15N]20 mM
7potassium chloridenatural abundance200 mM
8DTTnatural abundance1 mM
9H2Onatural abundance90 %
10D2Onatural abundance10 %
Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4


#NameIsotope labelingTypeConcentration
11HEPES[U-13C; U-15N]20 mM
12potassium chloridenatural abundance200 mM
13DTTnatural abundance1 mM
14D2Onatural abundance10 %

LACS Plot; CA
Referencing offset: -0.23 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.23 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.1 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.28 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2LBW, Strand ID: A Detail


Release date
2011-06-29
Citation 1
Structure of H/ACA RNP protein Nhp2p reveals cis/trans isomerization of a conserved proline at the RNA and Nop10 binding interface
Koo, B., Park, C., Fernandez, C.F., Chim, N., Ding, Y., Chanfreau, G., Feigon, J.
J. Mol. Biol. (2011), 411, 927-942, PubMed 21708174 , DOI 10.1016/j.jmb.2011.06.022 ,
Citation 2
Crystal structure of an H/ACA box ribonucleoprotein particle
Li, L., Ye, K.
Nature (2006), 443, 302-307, PubMed 16943774 , DOI 10.1038/nature05151 ,
Related entities 1. H/ACA RNP protein Nhp2p-S82W mutant, : 1 : 3 : 191 entities Detail
Interaction partners 1. H/ACA RNP protein Nhp2p-S82W mutant, : 41 interactors Detail
Experiments performed 10 experiments Detail
NMR combined restraints 5 contents Detail
Keywords L7Ae, Nhp2p, NMR solution structure, scaRNP, snoRNP