Solution structure of the RXLR effector P. capsici AVR3a4
GSSGSSGNVD SNQNKASMLQ ARLNDEAGGT RLLRVHHESD TEERGFLEKA AVKKMAKAIM ADPNKADEVY KKWADKGYTL TQMSNFLKSK TAGKYDRVYN GYVIHLDY
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 96.4 % (1189 of 1234) | 95.7 % (616 of 644) | 97.7 % (461 of 472) | 94.9 % (112 of 118) |
Backbone | 95.5 % (617 of 646) | 94.6 % (212 of 224) | 96.5 % (304 of 315) | 94.4 % (101 of 107) |
Sidechain | 97.4 % (669 of 687) | 96.2 % (404 of 420) | 99.2 % (254 of 256) | 100.0 % (11 of 11) |
Aromatic | 100.0 % (92 of 92) | 100.0 % (46 of 46) | 100.0 % (45 of 45) | 100.0 % (1 of 1) |
Methyl | 100.0 % (96 of 96) | 100.0 % (48 of 48) | 100.0 % (48 of 48) |
1. AVR3a4
GSSGSSGNVD SNQNKASMLQ ARLNDEAGGT RLLRVHHESD TEERGFLEKA AVKKMAKAIM ADPNKADEVY KKWADKGYTL TQMSNFLKSK TAGKYDRVYN GYVIHLDYSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | AVR3a4 | [U-100% 13C; U-100% 15N] | 1.11 mM | |
2 | TRIS | [U-100% 2H] | 20 mM | |
3 | sodium chloride | natural abundance | 300 mM | |
4 | DTT | [U-100% 2H] | 1 mM | |
5 | sodium azide | natural abundance | 0.02 % | |
6 | D2O | natural abundance | 10 % | |
7 | H2O | natural abundance | 90 % |