BMRB: 17590

Backbone assignments of Anabaena Sensory Rhodopsin Transducer with D-tails

Authors

Wang, S., Kim, S., Jung, K., Ladizhansky, V., Brown, L.S.

Assembly

Anabaena sensory rhodopsin transducer

Entity

1. Anabaena sensory rhodopsin transducer (polymer, Thiol state: all free), 124 monomers, 13730.14 Da Detail

SLSIGRTCWA IAEGYIPPYG NGPEPQFISH ETVCILNAGD EDAHVEITIY YSDKEPVGPY RLTVPARRTK HVRFNDLNDP APIPHDTDFA SVIQSNVPIV VQHTRLDSRQ AENALLSTIA YANT

Formula weight

13730.14 Da

Source organism

Trichormus variabilis

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 81.5 %, Completeness: 37.1 %, Completeness (bb): 65.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	37.1 % (520 of 1400)	19.7 % (142 of 721)	52.9 % (293 of 554)	68.0 % (85 of 125)
Backbone	65.4 % (472 of 722)	41.2 % (100 of 243)	78.4 % (287 of 366)	75.2 % (85 of 113)
Sidechain	17.6 % (140 of 796)	8.8 % (42 of 478)	32.0 % (98 of 306)	0.0 % (0 of 12)
Aromatic	1.8 % (2 of 110)	1.8 % (1 of 55)	1.9 % (1 of 54)	0.0 % (0 of 1)
Methyl	11.5 % (17 of 148)	10.8 % (8 of 74)	12.2 % (9 of 74)

1. ASRT

SLSIGRTCWA IAEGYIPPYG NGPEPQFISH ETVCILNAGD EDAHVEITIY YSDKEPVGPY RLTVPARRTK HVRFNDLNDP APIPHDTDFA SVIQSNVPIV VQHTRLDSRQ AENALLSTIA YANT

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
1	ASRT	[U-100% 13C; U-100% 15N; U-50% 2H]		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	Tris	natural abundance		50 mM
5	NaCl	natural abundance		50 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
6	ASRT	[U-100% 15N]		1 mM
7	H2O	natural abundance		90 %
8	D2O	natural abundance		10 %
9	Tris	natural abundance		50 mM
10	NaCl	natural abundance		50 mM

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LACS Plot; CA

Referencing offset: 0.51 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.51 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 1.23 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Release date

2011-06-21

Citation

A eukaryotic-like interaction of soluble cyanobacterial sensory rhodopsin transducer with DNA
Wang, S., Kim, S., Jung, K., Ladizhansky, V., Brown, L.S.
J. Mol. Biol. (2011), 411, 449-462, PubMed 21683709 , DOI 10.1016/j.jmb.2011.06.002 , Abstract

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. Anabaena sensory rhodopsin transducer, : 1 : 3 : 1 entities Detail

Experiments performed 8 experiments Detail

Chemical shift validation 3 contents Detail