Solution Structure of a Minor and Transiently Formed State of a T4 Lysozyme Mutant
MNIFEMLRID EGLRLKIYKD TEGYYTIGIG HLLTKSPSLN AAKSELDKAI GRNTNGVITK DEAEKLFNQD VDAAVRGILR NAKLKPVYDS LDAVRRAAAI NMVFQMGETG VAAFTNSLPM LQQKRWDEAA VNLAKSRWYN QTPNRAKRVI TTFRTGTWDA YKNL
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 53.5 % (1035 of 1933) | 38.2 % (384 of 1006) | 65.7 % (491 of 747) | 88.9 % (160 of 180) |
Backbone | 99.0 % (966 of 976) | 98.5 % (329 of 334) | 99.2 % (478 of 482) | 99.4 % (159 of 160) |
Sidechain | 20.1 % (223 of 1111) | 8.2 % (55 of 672) | 39.9 % (167 of 419) | 5.0 % (1 of 20) |
Aromatic | 5.8 % (8 of 138) | 8.7 % (6 of 69) | 3.0 % (2 of 66) | 0.0 % (0 of 3) |
Methyl | 14.8 % (29 of 196) | 7.1 % (7 of 98) | 22.4 % (22 of 98) |
1. T4 L99A/G113A/R119P
MNIFEMLRID EGLRLKIYKD TEGYYTIGIG HLLTKSPSLN AAKSELDKAI GRNTNGVITK DEAEKLFNQD VDAAVRGILR NAKLKPVYDS LDAVRRAAAI NMVFQMGETG VAAFTNSLPM LQQKRWDEAA VNLAKSRWYN QTPNRAKRVI TTFRTGTWDA YKNLSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 307 K, pH 5.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | T4 L99A/G113A/R119P | [U-100% 13C; U-100% 15N] | 1.5 mM | |
2 | H2O | natural abundance | 90 % | |
3 | D2O | natural abundance | 10 % | |
4 | NaCl | natural abundance | 25 mM | |
5 | EDTA | natural abundance | 2 mM | |
6 | NaN3 | natural abundance | 2 mM | |
7 | sodium phosphate | natural abundance | 50 mM |