Solution structure of the N-terminal domain of huntingtin (htt17) in presence of DPC micelles
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 57.5 % (65 of 113) | 57.5 % (65 of 113) |
Backbone | 88.2 % (30 of 34) | 88.2 % (30 of 34) |
Sidechain | 44.3 % (35 of 79) | 44.3 % (35 of 79) |
Aromatic | 40.0 % (4 of 10) | 40.0 % (4 of 10) |
Methyl | 55.6 % (5 of 9) | 55.6 % (5 of 9) |
1. htt17
MATLEKLMKA FESLKSFSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0, Details peptide after disaggregation
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | htt17 | 15N labeled at PHE11 | 0.2-0.3 mM | |
2 | D2O | natural abundance | 10 % | |
3 | sodium phosphate | natural abundance | 25 mM | |
4 | DPC | d38 | 100 mM | |
5 | H2O | natural abundance | 90 % |