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BMRB: 17646

2LD4

Solution structure of the N-terminal domain of human anamorsin

Authors

Banci, L., Bertini, I., Ciofi-Baffoni, S., Boscaro, F., Chatzi, A., Mikolajczyk, M., Tokatlidis, K., Winkelmann, J.

Assembly

Anamorsin

Entity

1. Anamorsin (polymer, Thiol state: all free), 172 monomers, 18516.93 Da Detail

MADFGISAGQ FVAVVWDKSS PVEALKGLVD KLQALTGNEG RVSVENIKQL LQSAHKESSF DIILSGLVPG STTLHSAEIL AEIARILRPG GCLFLKEPVE TAVDNNSKVK TASKLCSALT LSGLVEVKEL QREPLTPEEV QSVREHLGHE SDNLLFVQIT GKKPNFEVGS SR

Formula weight

18516.93 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 93.1 %, Completeness (bb): 98.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.1 % (1822 of 1956)	92.6 % (937 of 1012)	92.7 % (709 of 765)	98.3 % (176 of 179)
Backbone	98.4 % (1002 of 1018)	99.4 % (348 of 350)	97.6 % (491 of 503)	98.8 % (163 of 165)
Sidechain	89.2 % (978 of 1097)	89.0 % (589 of 662)	89.3 % (376 of 421)	92.9 % (13 of 14)
Aromatic	44.3 % (39 of 88)	77.3 % (34 of 44)	11.6 % (5 of 43)	0.0 % (0 of 1)
Methyl	98.2 % (224 of 228)	96.5 % (110 of 114)	100.0 % (114 of 114)

1. Anamorsin

MADFGISAGQ FVAVVWDKSS PVEALKGLVD KLQALTGNEG RVSVENIKQL LQSAHKESSF DIILSGLVPG STTLHSAEIL AEIARILRPG GCLFLKEPVE TAVDNNSKVK TASKLCSALT LSGLVEVKEL QREPLTPEEV QSVREHLGHE SDNLLFVQIT GKKPNFEVGS SR

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Anamorsin	[U-100% 15N]	0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance	50 mM
3	DTT	natural abundance	2 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	anamorsin	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 mM
7	sodium phosphate	natural abundance	50 mM
8	DTT	natural abundance	2 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	dioxane	methylene protons	61.5 ppm	external	indirect	0.2514495
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.27 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	dioxane	methylene protons	61.5 ppm	external	indirect	0.2514495
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.27 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	dioxane	methylene protons	61.5 ppm	external	indirect	0.2514495
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	dioxane	methylene protons	61.5 ppm	external	indirect	0.2514495
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.21 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 30 models in PDB: 2LD4, Strand ID: A Detail

Release date

2011-06-29

Citation

Anamorsin is a [2Fe-2S] cluster-containing substrate of the Mia40-dependent mitochondrial protein trapping machinery
Banci, L., Bertini, I., Ciofi-Baffoni, S., Boscaro, F., Chatzi, A., Mikolajczyk, M., Tokatlidis, K., Winkelmann, J.
Chem. Biol. (2011), 18, 794-804, PubMed 21700214 , DOI 10.1016/j.chembiol.2011.03.015 , Abstract

Entries sharing articles Swiss-Prot: 2 entries Detail

  Swiss-Prot: Q6FI81 released on 2005-01-04
    Title CPIN1_HUMAN Entity Anamorsin
  Swiss-Prot: P36152 released on 1994-06-01
    Title DRE2_YEAST Entity Fe-S cluster assembly protein DRE2

Related entities 1. Anamorsin, : 1 : 3 : 1 : 26 entities Detail

Interaction partners 1. Anamorsin, : 9 interactors Detail

More details for 9 interactors identified by 6 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC