Solution NMR Structure of Heat shock factor protein 1 DNA binding domain from homo sapiens, Northeast Structural Genomics Consortium Target HR3023C
MGHHHHHHSH MAGPSNVPAF LTKLWTLVSD PDTDALICWS PSGNSFHVFD QGQFAKEVLP KYFKHNNMAS FVRQLNMYGF RKVVHIEQGG LVKPERDDTE FQHPCFLRGQ EQLLENIKRK VTSVS
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 90.9 % (1362 of 1499) | 91.2 % (712 of 781) | 90.8 % (531 of 585) | 89.5 % (119 of 133) |
Backbone | 90.1 % (663 of 736) | 90.0 % (226 of 251) | 90.5 % (332 of 367) | 89.0 % (105 of 118) |
Sidechain | 91.8 % (808 of 880) | 91.7 % (486 of 530) | 91.9 % (308 of 335) | 93.3 % (14 of 15) |
Aromatic | 81.6 % (142 of 174) | 83.9 % (73 of 87) | 80.0 % (68 of 85) | 50.0 % (1 of 2) |
Methyl | 100.0 % (112 of 112) | 100.0 % (56 of 56) | 100.0 % (56 of 56) |
1. HR3023C
MGHHHHHHSH MAGPSNVPAF LTKLWTLVSD PDTDALICWS PSGNSFHVFD QGQFAKEVLP KYFKHNNMAS FVRQLNMYGF RKVVHIEQGG LVKPERDDTE FQHPCFLRGQ EQLLENIKRK VTSVSSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.51 mM [U-100% 13C; U-100% 15N], 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR3023C | [U-100% 13C; U-100% 15N] | 0.51 mM | |
2 | H2O | natural abundance | 90 % | |
3 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details NMR buffer 6.5 -- 0.02% NaN3, 10mM DTT, 5mM CaCL2, 100mM NaCL, 1x Proteinase Inhibitors, 20mM MES pH 6.5, 10% D2O, 50uM DSS
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | HR3023C | [U-100% 13C; U-100% 15N] | 0.43 mM | |
5 | NaN3 | natural abundance | 0.02 % | |
6 | DTT | natural abundance | 10 mM | |
7 | CaCL2 | natural abundance | 5 mM | |
8 | NaCL | natural abundance | 100 mM | |
9 | Proteinase Inhibitors | natural abundance | 1 na | |
10 | MES | natural abundance | 20 mM | |
11 | DSS | natural abundance | 50 uM | |
12 | D2O | natural abundance | 10 % | |
13 | H2O | natural abundance | 90 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details NMR buffer 6.5 -- 0.02% NaN3, 10mM DTT, 5mM CaCL2, 100mM NaCL, 1x Proteinase Inhibitors, 20mM MES pH 6.5, 10% D2O, 50uM DSS aligned in Positively Charged Compressed Poly Acrylamide Gel.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
14 | HR3023C | [U-100% 13C; U-100% 15N] | 0.43 mM | |
15 | NaN3 | natural abundance | 0.02 % | |
16 | DTT | natural abundance | 10 mM | |
17 | CaCL2 | natural abundance | 5 mM | |
18 | NaCL | natural abundance | 100 mM | |
19 | Proteinase Inhibitors | natural abundance | 1 na | |
20 | MES | natural abundance | 20 mM | |
21 | DSS | natural abundance | 50 uM | |
22 | D2O | natural abundance | 10 % | |
23 | H2O | natural abundance | 90 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details NMR buffer 6.5 -- 0.02% NaN3, 10mM DTT, 5mM CaCL2, 100mM NaCL, 1x Proteinase Inhibitors, 20mM MES pH 6.5, 10% D2O, 50uM DSS aligned in Peg/hexanol.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
24 | HR3023C | [U-100% 13C; U-100% 15N] | 0.43 mM | |
25 | NaN3 | natural abundance | 0.02 % | |
26 | DTT | natural abundance | 10 mM | |
27 | CaCL2 | natural abundance | 5 mM | |
28 | NaCL | natural abundance | 100 mM | |
29 | Proteinase Inhibitors | natural abundance | 1 na | |
30 | MES | natural abundance | 20 mM | |
31 | DSS | natural abundance | 50 uM | |
32 | D2O | natural abundance | 10 % | |
33 | H2O | natural abundance | 90 % |