Novel dimeric structure of phage phi29-encoded protein p56: Insights into Uracil-DNA glycosylase inhibition
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 92.6 % (375 of 405) | 92.2 % (317 of 344) | 95.1 % (58 of 61) |
Backbone | 96.5 % (165 of 171) | 97.4 % (112 of 115) | 94.6 % (53 of 56) |
Sidechain | 89.7 % (210 of 234) | 89.5 % (205 of 229) | 100.0 % (5 of 5) |
Aromatic | 56.3 % (18 of 32) | 56.3 % (18 of 32) | |
Methyl | 100.0 % (33 of 33) | 100.0 % (33 of 33) |
1. p56
MVQNDFVDSY DVTMLLQDDD GKQYYEYHKG LSLSDFEVLY GNTADEIIKL RLDKVLSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | p56 | natural abundance | 0.5 mM | |
2 | sodium chloride | natural abundance | 100 mM | |
3 | potassium phosphate | natural abundance | 10 mM | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 90 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | p56 | [U-98% 13C; U-98% 15N] | 0.5 mM | |
7 | sodium chloride | natural abundance | 100 mM | |
8 | potassium phosphate | natural abundance | 10 mM | |
9 | H2O | natural abundance | 90 % | |
10 | D2O | natural abundance | 90 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
11 | p56 | natural abundance | 0.5 mM | |
12 | sodium chloride | natural abundance | 100 mM | |
13 | potassium phosphate | natural abundance | 10 mM | |
14 | H2O | natural abundance | 90 % | |
15 | D2O | natural abundance | 90 % |