Human Insulin Mutant A22Gly-B31Arg
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS6:SG | 1:CYS11:SG |
2 | disulfide | sing | 1:CYS7:SG | 2:CYS7:SG |
3 | disulfide | sing | 1:CYS20:SG | 2:CYS19:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | |
---|---|---|---|
All | 81.4 % (451 of 554) | 96.2 % (302 of 314) | 62.1 % (149 of 240) |
Backbone | 77.3 % (204 of 264) | 96.4 % (106 of 110) | 63.6 % (98 of 154) |
Sidechain | 86.1 % (291 of 338) | 96.1 % (196 of 204) | 70.9 % (95 of 134) |
Aromatic | 48.6 % (34 of 70) | 97.1 % (34 of 35) | 0.0 % (0 of 35) |
Methyl | 98.2 % (55 of 56) | 96.4 % (27 of 28) | 100.0 % (28 of 28) |
1. InsulinGR 1
GIVEQCCTSI CSLYQLENYC NG2. InsulinGR 2
FVNQHLCGSH LVEALYLVCG ERGFFYTPKT RSolvent system 65% H2O / 35% CD3CN, Pressure 1 atm, Temperature 298 K, pH 3.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | InsulinGR 1 | natural abundance | 2.0 ~ 3.0 mM | |
2 | InsulinGR 2 | natural abundance | 2.0 ~ 3.0 mM | |
3 | H2O | natural abundance | 65 % | |
4 | CD3CN | natural abundance | 35 % |
Solvent system 65% D2O / 35% CD3CN, Pressure 1 atm, Temperature 298 K, pH 3.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | InsulinGR 1 | natural abundance | 2.0 ~ 3.0 mM | |
6 | InsulinGR 2 | natural abundance | 2.0 ~ 3.0 mM | |
7 | D2O | natural abundance | 65 % | |
8 | CD3CN | natural abundance | 35 % |