Human C30S/C59S-Cox17 mutant
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 70.1 % (534 of 762) | 57.5 % (233 of 405) | 83.4 % (246 of 295) | 88.7 % (55 of 62) |
Backbone | 88.9 % (345 of 388) | 87.9 % (116 of 132) | 88.8 % (174 of 196) | 91.7 % (55 of 60) |
Sidechain | 56.4 % (246 of 436) | 42.9 % (117 of 273) | 80.1 % (129 of 161) | 0.0 % (0 of 2) |
Aromatic | 0.0 % (0 of 32) | 0.0 % (0 of 16) | 0.0 % (0 of 16) | |
Methyl | 55.8 % (29 of 52) | 11.5 % (3 of 26) | 100.0 % (26 of 26) |
1. Human C30S/C59S-Cox17 mutant
GSFTMPGLVD SNPAPPESQE KKPLKPCCAS PETKKARDAC IIEKGEEHCG HLIEAHKESM RALGFKISolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | C30S/C59S-Cox17_mutant | [U-100% 15N] | 0.5 mM | |
2 | sodium phosphate | natural abundance | 50 mM | |
3 | EDTA | natural abundance | 0.5 mM | |
4 | DTT | natural abundance | 1 mM | |
5 | D2O | natural abundance | 10 % | |
6 | H2O | natural abundance | 90 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | C30S/C59S-Cox17_mutant | [U-99% 13C; U-99% 15N] | 1 mM | |
8 | sodium phosphate | natural abundance | 50 mM | |
9 | EDTA | natural abundance | 0.5 mM | |
10 | DTT | natural abundance | 1 mM | |
11 | D2O | natural abundance | 10 % | |
12 | H2O | natural abundance | 90 % |