Syrian hamster prion protein with thiamine
LGGYMLGSAM SRPMMHFGND WEDRYYRENM NRYPNQVYYR PVDQYNNQNN FVHDCVNITI KQHTVTTTTK GENFTETDIK IMERVVEQMC TTQYQKESQA YYDG
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS55:SG | 1:CYS90:SG |
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 37.1 % (286 of 770) | 28.5 % (185 of 650) | 84.2 % (101 of 120) |
Backbone | 91.0 % (284 of 312) | 86.7 % (183 of 211) | 100.0 % (101 of 101) |
Sidechain | 0.4 % (2 of 458) | 0.5 % (2 of 439) | 0.0 % (0 of 19) |
Aromatic | 0.0 % (0 of 68) | 0.0 % (0 of 67) | 0.0 % (0 of 1) |
Methyl | 2.6 % (1 of 38) | 2.6 % (1 of 38) |
1. shPrP
LGGYMLGSAM SRPMMHFGND WEDRYYRENM NRYPNQVYYR PVDQYNNQNN FVHDCVNITI KQHTVTTTTK GENFTETDIK IMERVVEQMC TTQYQKESQA YYDGSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.2, Details 300 M, 350 L, 20 mM KH2PO4, pH 6.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | shPrP | [U-100% 15N] | 0.3 mM | |
2 | thiamine | natural abundance | 10 mM | |
3 | KH2PO4 | natural abundance | 20 mM | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | shPrP | natural abundance | 0.5 mM | |
7 | thiamine | natural abundance | 12.5 mM | |
8 | H2O | natural abundance | 90 % | |
9 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | shPrP | natural abundance | 0.5 mM | |
11 | thiamine | natural abundance | 10 mM | |
12 | H2O | natural abundance | 90 % | |
13 | D2O | natural abundance | 10 % |